反复呼吸道感染患儿细胞及体液免疫功能分析  

Analysis of cellular and humoral immunity of children with recurrent respiratory tract infections

作　　者：李小青[1] 段明玥[1] 李丹[1] 张晰[1] 

机构地区：[1]西安交通大学医学院附属儿童医院免疫科,陕西西安710003

出　　处：《中国妇幼健康研究》2013年第4期469-471,共3页Chinese Journal of Woman and Child Health Research

基　　金：陕西省卫生厅科学研究基金资助项目(2010D24)

摘　　要：目的分析反复呼吸道感染患儿细胞及体液免疫功能。方法选择2010年10月至2012年9月确诊为反复呼吸道感染患儿60例,按照年龄将其分为:A1组年龄<3岁,B1组3~6岁;同时选择健康儿童60例作为对照组:A2组年龄<3岁,B2组3~6岁。采用流式细胞术检测所有入组儿童外用血T淋巴细胞总数(CD^(3+))及其亚群(CD^(3+)CD^(4+)、CD^(3+)CD^(8+))的绝对计数;速率散射比浊法测定其血清免疫球蛋白G、A、M(IgG、IgA、IgM)含量。结果 A1、B1组CD^(3+)、CD^(3+)CD^(4+)细胞绝对计数及IgG含量均较相应正常对照组A2、B2组降低,差异均有统计学意义(A1与A2组比较t值分别为0.003、0.033、0.017;B1与B2组比较t值分别为0.041、0.045、0.012,均P<0.05)。在<3岁儿童中,反复呼吸道感染患儿的IgA含量较正常同龄儿童偏低,差异有统计学意义(t=0.035,P<0.05)。结论反复呼吸道感染患儿CD^(3+)T淋巴细胞总数绝对计数的降低,尤其是CD^(3+)CD^(4+)T辅助细胞的减少,使其对T、B淋巴细胞增殖分化的调控作用削弱,浆细胞产生Ig过程障碍是导致儿童反复呼吸道感染的重要原因。Objective To analyze the cellular and humoral immunity of children affected by recurrent respiratory tract infections（RRTI）. Methods Sixty children diagnosed with RRTI during the period of October 2010 to September 2012 were divided into A1 group（ 〈 3 years of age） and B1 group （ 3-6 years of age）. Meanwhile, 60 healthy children were taken as control group ： A2 group （ 〈 3 years of age） and B2 group （3-6 years of age）. The absolute count of CD3＋ T lymphocyte as well as CD3＋ CD4＋ and CD3＋ CDs＋ subsets of enrolled children were detected by flow cytometry, and the serum content of immunoglobulin G, A and M （IgG, IgA and IgM ） was also determined by rate nephelometry. Results The absolute counts of CD3 ＋ and CD3 ＋ CD4 ＋ and IgG content both in A1 and B1 group were observed to be lower than the corresponding counts and content in A2 and B2 group, and there were statistical differences between patients and control groups （ comparing A1 with A2 group, t value was 0. 003, 0. 033 and 0. 017, respectively; comparing B1 with B2 group, t value was 0. 041, 0. 045 and 0.012, respectively, all P 〈 0.05 ）. When children were under 3 years old, those with RRTI had lower content of IgA than healthy children with same age, and the difference was significant （ t = 0. 035, P 〈 0.05）. Conclusion The decrease of absolute count of CD3 ＋T lymphocyte, especially the decrease of CD3＋ CD4＋T helper cell, weakens the regulatory function on proliferation and differentiation of T and B lymphocyte, which leads to obstruction of Ig molecules production. That is the important reason for children RRTI.

关 键 词：反复呼吸道感染 细胞免疫 体液免疫 儿童 

分 类 号：R725.6[医药卫生—儿科]