烟碱降解菌CW6菌株的分离鉴定及其降解特性  

Isolation and characterization of a nicotine-degrading bacterium CW6strain

作　　者：翟贵发[1] 陈素卉[1] 李涵[1] 王映月[1] 杨晓潼[1] 王晓丽 郭利 李晓华[1] 

机构地区：[1]中南民族大学生命科学学院生物技术国家民委重点实验室,武汉430074 [2]湖北省襄阳市烟草专卖局,湖北襄阳441003

出　　处：《华中师范大学学报（自然科学版）》2013年第5期676-680,共5页Journal of Central China Normal University：Natural Sciences

基　　金：国家自然科学基金项目(31070087;30570046);湖北省自然科学基金重点项目(2011CDA079);国家大学生创新创业训练项目(GCX12012);湖北省烟草公司面上项目(0710XYYC2012-01)

摘　　要：以烟碱为唯一碳源,从湖北省襄阳市烟叶种植土壤中分离得到1株烟碱降解菌,命名为CW6,经常规的形态观察、生理生化分析以及16SrDNA序列同源性分析,初步鉴定该菌株为假单胞菌属(Pseudomonas).CW6菌株降解烟碱最适温度为30℃.当烟碱质量浓度为2.0g/L时,烟碱降解率达到88.18%.当烟碱质量浓度达到5.5g/L时,烟碱降解率达到45.83%.当烟碱质量浓度高于6.5g/L时,菌株生长和烟碱降解率均呈下降趋势,烟碱降解率低于8.53%.CW6菌株在烟碱质量浓度为2.0g/L的烟碱培养基中培养,不同时间样品紫外扫描显示:0h在259nm处有吸收波峰,A259值为0.9946,当培养时间达到48h时,259nm处烟碱特征吸收峰降低至0.1370.A nicotine-degrading bacterium,strain CW6,was isolated from the tobacco field soil,using nicotine as the sole source of carbon.Based on standard morphological,physiological properties and 16S rRNA gene sequence,it was identified as Pseudomonas sp..With initial nicotine concentration of 2.0 g/L,the strain CW6 grew well and degraded 88.18％ of nicotine.With 5.5 g/L nicotine,45.83％ of nicotine was degraded.Both the growth and degradation declined with the degration of less than 8.53 ％ when the nicotine concentration was more than 6.5 g/L.UV scanning of strain CW6 cultured in 2.0 g/L nicotine at different time showed that single absorption peak existed at 259 nm in 0 h,peak height decreased at 259 nm gradually.

关 键 词：烟碱 16S RDNA 生物降解 假单胞菌 

分 类 号：Q939[生物学—微生物学]