NIV毒素对软骨细胞骨架蛋白的影响及硒的拮抗作用  

NIV toxin on cytoskeletal proteins in cartilage and the Antagonism of selenium

作　　者：李锦[1] 王加丽[1] 罗明秀[1] 曹峻岭[1] 

机构地区：[1]西安交通大学医学院地方病研究所,陕西西安710061

出　　处：《国外医学（医学地理分册）》2013年第3期146-150,共5页Foreign Medical Sciences：Section of Medgeography

基　　金：国家自然科学基金资助项目(No.31070725;30872187;30471499;30170831)

摘　　要：目的利用C-28/12软骨细胞系进行体外单层培养,研究KBD可疑致病因子雪腐镰刀菌烯醇和硒对软骨细胞骨架蛋白中肌动蛋白和波形蛋白的影响。方法用Real-time PCR法检测细胞中肌动蛋白和波形蛋白mRNA的表达;用Western-blot方法检测细胞中肌动蛋白和波形蛋白蛋白的表达。结果①肌动蛋白在毒素组和毒素加硒组均表现为随着毒素浓度的升高mRNA的表达逐渐升高;毒素组肌动蛋白蛋白的表达量随着毒素浓度的升高而逐渐降低,毒素加硒组蛋白的表达量与相应的毒素组相比较,可显示出硒的拮抗作用。②波形蛋白PCR的结果:毒素组表现为随着毒素浓度的增加有下降的趋势,毒素加硒组表现为随着毒素浓度的增加有逐渐升高的趋势;波形蛋白Western-blot的结果:毒素组表现为随着毒素浓度的增加蛋白的表达逐渐下降,毒素加硒组表现为随着毒素浓度的增加有升高的趋势。结论 NTV毒素对肌动蛋白和波形蛋白mRNA和蛋白的表达有影响。Objective C-28/I2 chondrocyte cell line ~vas monolayerly cultured in vitro to study the effect of NIV and selenium on the actin and vimentin. Methods The mRNA expressions of actin and vimentin were detected with real-time PCR. The protein expressions of actin and vimentin were determined with Western-blot method. Results In both the toxin and toxin plus selenium groups, the mRNA expression of actin increased with the toxin concentration, while in the toxin group, the protein expression of actin decreased with the toxin concentration,indicating the antagonism of selenium. The mRNA expression and protein expression of vimentin decreased with the increase of toxin concentration in the toxin group, but elevated in the toxin plus selenium group. Conclusion Toxin has effect on the mRNA and protein expressions of actin and vimentin.

关 键 词：大骨节病 雪腐镰刀菌烯醇 硒 骨架蛋白 

分 类 号：R684.1[医药卫生—骨科学]