猪PLIN1基因单核苷酸多态检测与遗传分析  

Single nucleotide polymorphism detection and genetic analysis of porcine PLIN1 gene

作　　者：黄龙[1] 余大华 何小雷[1] 朱卫华[1] 孟云[1] 薛玮玮[1] 丁月云[1] 殷宗俊[1] 

机构地区：[1]安徽农业大学动物科技学院,安徽地方畜禽遗传资源保护与生物育种省级实验室,合肥230036 [2]安徽安泰农业集团,广德242200

出　　处：《安徽农业大学学报》2013年第5期701-705,共5页Journal of Anhui Agricultural University

基　　金：国家自然科学基金(31171200);科技富民强县专项行动计划;国家农业科技成果转化资金(2011GB2C300017);省级农业科技专家大院(2012);科技部星火计划重点项目(2010GA710001)共同资助

摘　　要：以皖南黑猪、安庆六白猪、圩猪和霍寿黑猪共236头猪为研究对象,采用PCR-SSCP方法检测猪脂滴包被蛋白(Perilipin,PLIN1)基因的遗传多态性。结果表明,在皖南黑猪、安庆六白猪PLIN1基因3 519 bp碱基处发生A>G突变,检测到AA、AB、BB 3种基因型,其中AA基因型频率在这2个群体中分别为8.97%和29.23%,AB基因型频率分别为43.59%和56.92%,BB基因型频率分别为47.44%和13.85%;皖南黑猪、安庆六白猪的多态信息含量(PIC)分别为0.335 3和0.369 0,在该位点均处于中度多态;卡方检验表明皖南黑猪群体和安庆六白猪群体均处于哈迪-温伯格平衡状态(P>0.05);在圩猪、霍寿黑猪群体中PLIN1基因的第2外显子没有检测到突变。PLIN1基因的第3外显子在4个群体中均没有检测到突变。Two hundred and thirty-six pigs from Wannan Black pig, Anqing Liubai pig, Wei Pig and Huoshou Black pig populations were used for detecting the polymorphism of PLIN1 gene by PCR-SSCP method. The results showed that a A〉G mutation at 3 519 base position, and 3 genotypes （AA, AB and BB） were found in Wannan Black and Anqing Liubai. The genotypic frequency of AA was 8.97%, 29.23%, respectively, AB was 43.59%, 56.92%, respectively, and BB was 47.44%, 13.85%, respectively. The polymorphism information content （PIC） was 0.335 3 in Warman Black population and 0.369 0 in Anqing Liubai population, which were all in aver- age polymorphism situation. According to the Z2 test, there were in Hardy-Weinberg equilibrium in Wannan Black and Anqing Liubai population （P〉0.05）, but there was no mutation in exon 2 in Wei Pig and Huoshou Black pig populations. Moreover, there was no mutation in exon 3 in these four populations.

关 键 词：猪 PLIN1基因 单核苷酸多态性 遗传分析 

分 类 号：S828.2[农业科学—畜牧学]