不同分离方法获得的冬虫夏草无性型菌株的交配型基因MAT1-2-1的初步分析  

Preliminary analysis of MAT1-2-1 mating type genes of anamorphic isolates from different sources of Ophiocordyceps sinensis

作　　者：张磊[1] 肖岩岩[1] 陈超[1] 李春如[1] 

机构地区：[1]安徽农业大学微生物防治省重点实验室,合肥230036

出　　处：《安徽农业大学学报》2013年第5期819-822,共4页Journal of Anhui Agricultural University

基　　金：国家高技术研究发展计划(863计划)(2007AA021506);教育部留学回国人员科研启动基金(2009年)共同资助

摘　　要：对采用不同分离方法获得的40个冬虫夏草(Ophiocordyceps sinensis)无性型菌株进行交配型基因MAT1-2-1的PCR特异扩增,并随机取其中7个拼接好的MAT1-2-1的碱基序列,与4个来自GenBank数据在内的共11个样品构建了系统发育树。结果表明,采自青海的7个供试菌株,连同GenBank中来自青海的菌株(FJ654176)共同组成了一大类群,而GenBank中其他地区的菌株则聚成另外的类群;随机分离得到的10个单子囊孢子菌株全部含有MAT1-2-1基因,MAT1-1:MAT1-2未见按4:4进行分离,提示冬虫夏草极有可能是同宗配型。There is close relationship between the mating type gene of Ophiocordyceps sinensis and the arti- ficial introduction of fruiting body. Many scholars focus on the mating type of O.sinensis, and MAT1-2-1 sequences of 40 isolates of O.sinensis by different methods were amplified with the primers MAT1-2-1 and evolutionary phylogenetic analyses were performed with sequences of the MAT1-2-1 gene, of which 7 sequences of MAT1-2-1 came from this experiment randomly and 4 sequences of MAT1-2-1 cited from the GenBank data. The result showed that 7 isolates collected from Qinghai, together with the isolates from Qinghai in GenBank make up a large group with the 96% bootstrap value and the isolates from the other area clustered into the other part. Meanwhile, all of single ascospore isolates of O.sinensis contain the MAT1-2-1 gene and the ratio of MAT l-l： MAT1-2 has not been found by 4：4. So O. sinensis might not be heterothallism, to a large extent, it is homothallic.

关 键 词：冬虫夏草 无性型菌株 MAT1 2 1基因 单子囊孢子 同宗配型 

分 类 号：Q949.32[生物学—植物学]