257份小麦品种资源中矮秆基因的分子检测  

Detection of dwarf genes in 257 wheat variety resources using molecular markers

作　　者：王玉叶[1,2] 张海萍[1] 来得娥[1] 赵秋霞[1] 常成[1] 马传喜[1] 

机构地区：[1]农业部黄淮南部小麦生物学与遗传育种区域重点实验室,安徽省作物生物学重点实验室,安徽农业大学农学院,合肥230036 [2]安徽省淮南农场农业科学研究所,淮南232035

出　　处：《安徽农业大学学报》2013年第5期860-866,共7页Journal of Anhui Agricultural University

基　　金：农业部行业科技专项资金;安徽省小麦产业技术体系专项资金共同资助

摘　　要：矮秆基因Rht-B1b、Rht-D1b和Rht8等的广泛利用,不仅增强了小麦的抗倒性,而且提高了产量。明确矮秆基因的分布,可以为小麦矮化育种提供分子信息。采用STS和SSR标记检测257份小麦品种资源中Rht-B1b、Rht-D1b和Rht8基因的分布情况。结果表明,257份材料中,Rht8基因分布频率最高(106个品种,41.2%),Rht-D1b次之(88个品种,34.2%),Rht-B1b最低(70个品种,27.2%)。此外,部分材料中含有不同类型的矮秆基因组合,且分布频率不同,其中Rht-D1b+Rht8(25个品种,9.7%)>Rht-B1b+Rht8(24个品种,9.3%)>Rht-B1b+Rht-D1b(9个品种,3.5%)>Rht-B1b+Rht-D1b+Rht8(5个品种,1.9%)。上述结果为小麦抗倒伏育种以及矮化育种提供了重要的参考信息。Extensive use ofRht-Blb, Rht-Dlb and Rht8 dwarf genes can not only enhance wheat lodging resistance, but also increase wheat yield. Clear distribution dwarf genes will provide molecular information in wheat dwarf breeding. In this article, we detected the distribution of Rht-Bla, Rht-Blb, Rht-Dlb and Rht8 gene in 257 wheat varieties using STS and SSR molecular markers. The results showed that the frequency ofRht8 gene （106 wheat varieties, 41.2%） was the highest, Rht-Dlb was higher （88 wheat varieties, 34.2%） and Rht-Blb was the lowest （70 wheat varieties, 27.2%） in 257 wheat resources. The research also showed that part of wheat resources contained different kinds of dwarfing gene combination, and their distribution frequencies were also different. Twenty five materials （9.7%） were detected to contain Rht-Dlb and Rht8. Twenty four materials （9.3%） were de- tected to have Rht-Blb and Rht8. Nine materials （3.5%） were detected to carry Rht-Dlb and Rht-Blb. Five mate- rials （1.9%） were detected to contain Rht-Blb, Rht-Dlb and Rht8. The results may provide important information for wheat lodging resistance breeding and dwarfing breeding.

关 键 词：小麦 分子标记 矮秆基因 

分 类 号：S512.1[农业科学—作物学]