石竹(Dianthus chinensis)对土壤镉胁迫的生理生化响应  

Physiological and Biochemical Responses of Dianthus chinensis to Soil Cadmium Stress

作　　者：贺靖舒[1] 丁继军[1,2] 潘远智[1] 刘柿良[1] 李丽[3] 

机构地区：[1]四川农业大学风景园林学院,四川温江611130 [2]长江三峡实业有限公司,湖北宜昌443002 [3]西南林业大学林学院,昆明650224

出　　处：《四川农业大学学报》2013年第3期290-295,327,共7页Journal of Sichuan Agricultural University

基　　金：四川省教育厅重点项目(09ZA065)

摘　　要：【目的】为探讨石竹对土壤重金属Cd污染的抗性和敏感性。【方法】采用盆栽控制试验,测定不同浓度(0、0.3、1、3、10、30、50mg/kg)Cd胁迫下石竹叶片生理生化指标。【结果】研究表明,石竹根重、地上部干重、株高、抗性系数以及可溶性蛋白含量以3mg/kg Cd浓度为阈值呈低促高抑现象。随Cd处理浓度、时间的增加,游离脯氨酸、丙二醛含量和质膜相对透性均逐渐上升,且在高浓度(≥10mg/kg)下上升更为显著;SOD、POD和CAT酶活性呈先上升后下降趋势,上升最大值的处理浓度分别为10、3、3mg/kg。【结论】低浓度Cd对石竹影响较小,当浓度大于10mg/kg时,其毒性随浓度和时间的增加而明显增大,表明石竹对土壤Cd胁迫有一定适应性。[Objective] The aim of this study was to investigate the resistances and susceptibility of D. chinensis to soil heavy metal cadmium （Cd）. [Method] A pot-experiment was conducted to measure physiological and biochemical indicators of D. chinensis leaf grown in different Cd concen- trations （0, 0.3, 1, 3, 10, 30 and 50 mg/kg）. [Results] The biomass of root and shoot, plant height, resistance coefficient and content of soluble protein, increased when Ca concentration was below 3 mg/kg but the opposite was true when Ca concentration was above 3 mg/kg. With the in crease of cadmium concentrations and treatment time, free proline, malondialdehyde and relative conductivity increased gradually. The activities of SOD, POD and CAT increased initially and then declined. [Conclusion] Low Cd concentrations had little effects on D. chinensis when Cd con centration 10 mg/kg, the toxicity of cadmium was increased with an increase in concentration and time, implying that D. chinensis is relatively adaptable to soil cadmium stress.

关 键 词：CD胁迫 石竹 生理生化 响应 

分 类 号：Q945.78[生物学—植物学]