Genetic Analysis and Molecular Mapping of Novel White Striped Leaf Mutant Gene in Rice  

作　　者：SHENG Zhong-hua WEI Xiang-jin SHAO Gao-neng SONG Jian LUO Ju HU Pei-song TANG Shao-qing CHEN Li-yun 

机构地区：[1]Rice Research Institute, Hunan Agricultural University [2]China National Rice Research Institute

出　　处：《Rice science》2013年第5期336-342,共7页水稻科学（英文版）

基　　金：supported by the grants from the National High Technology Research and Development Program of China(Grant No.2011AA10A101);the Natural Science Foundation of Zhejiang Province of China(Grant No.Y12C13003);the National Natural Science Foundation of China(Grant No.31201193)

摘　　要：A new white striped leaf mutant wsll was discovered from Nipponbare mutated by ethyl methanesulfonate. The mutant showed white striped leaves at the seedling stage and the leaves gradually turned green after the tillering stage. The chlorophyll content of wsll was significantly lower than that of wild-type during the fourth leaf stage, tillering stage and booting stage. The numbers of chloroplast, grana and grana lamella were reduced and the thylakoids were degenerated in wsll compared with wild type. Genetic analysis showed that the wsll was controlled by a single recessive gene. Molecular mapping of the wsll was performed using an F2 population derived from wsll/Nanjing 11. The wsll was finally mapped on the telomere region of chromosome 9 and positioned between simple sequence repeat markers RM23742 and RM23759 which are separated by approximately 486.5 kb. The results may facilitate map-based cloning of wsll and understanding of the molecular mechanism of the regulation of leaf-color by WSL1 in rice.A new white striped leaf mutant wsll was discovered from Nipponbare mutated by ethyl methanesulfonate. The mutant showed white striped leaves at the seedling stage and the leaves gradually turned green after the tillering stage. The chlorophyll content of wsll was significantly lower than that of wild-type during the fourth leaf stage, tillering stage and booting stage. The numbers of chloroplast, grana and grana lamella were reduced and the thylakoids were degenerated in wsll compared with wild type. Genetic analysis showed that the wsll was controlled by a single recessive gene. Molecular mapping of the wsll was performed using an F2 population derived from wsll/Nanjing 11. The wsll was finally mapped on the telomere region of chromosome 9 and positioned between simple sequence repeat markers RM23742 and RM23759 which are separated by approximately 486.5 kb. The results may facilitate map-based cloning of wsll and understanding of the molecular mechanism of the regulation of leaf-color by WSL1 in rice.

关 键 词：RICE white striped leaf mutant genetic analysis gene mapping 

分 类 号：S511[农业科学—作物学] Q5[生物学—生物化学]