芝麻素抗肿瘤作用的研究  

Study of the effect of the sesamin on anti-tumor

作　　者：张东旭[1] 范引科[2] 郭淑云[3] 李穆琼[1] 李晓晔[1] 刘雪英[1] 

机构地区：[1]第四军医大学药学系药物化学与药物分析学教研室,陕西西安710032 [2]陕西省中医药研究院,陕西西安710003 [3]第四军医大学附属唐都医院药剂科,陕西西安710038

出　　处：《中国医药导报》2013年第29期101-104,共4页China Medical Herald

摘　　要：目的通过抑瘤试验、延长生命试验、免疫试验及增效减毒试验进一步验证芝麻素的作用,为其进入临床提供实验依据。方法建立S180荷瘤小鼠移植瘤模型,随机分为荷瘤组、环磷酰胺(CTX)40 mg/kg组和芝麻素(1.0、0.5、0.25 g/kg)组,连续给药10 d,检测各组抑瘤率和生命延长率;建立S180荷瘤小鼠移植瘤模型,随机分为荷瘤组、芝麻素(0.5 g/kg)组、芝麻素(0.25 g/kg)组、CTX(10 mg/kg)组、5氟尿嘧啶(5-FU)20 mg/kg组、芝麻素(0.5、0.25 g/kg)+CTX(10 mg/kg)组和芝麻素(0.5、0.25 g/kg)+5-FU(20 mg/kg)组,分别连续给药10 d,检测各组抑瘤率;建立S180荷瘤小鼠移植瘤模型,随机分为荷瘤组、CTX(40 mg/kg)组、芝麻素(1.0、0.5、0.25 g/kg)+CTX(40 mg/kg)组,分别连续给药10 d,检测各组小鼠外周血白细胞(WBC)总数、细胞总数和抑瘤率。结果芝麻素1.0 g/kg和0.5 g/kg剂量能明显抑制小鼠移植性S180瘤体的生长,抑瘤率分别为28.40%、25.80%;芝麻素(1.0、0.5、0.25 g/kg)组对荷S180腹水瘤小鼠的平均生命延长率分别为32.24%、24.18%、17.96%;芝麻素(0.5、0.25 g/kg)+CTX(10 mg/kg)组和芝麻素(0.5、0.25 g/kg)+5-FU(20 mg/kg)组具有明显的抑瘤作用,抑瘤率(73.77%、71.92%和75.41%、73.15%)均大于两者合并用药后的理论相加效应值(71.72%、68.10%和74.96%、71.74%);芝麻素(1.0、0.5 g/kg)组可使骨髓有核细胞数明显回升(均P<0.05),各芝麻素组均可使外周血白细胞数有明显的回升,差异有统计学意义(P<0.05)。结论芝麻素具有抗肿瘤作用,与CTX或5-FU联合应用于肿瘤治疗,具有明显的协同增效作用和减毒作用。Objective To provide experimental evidence for sesamin entering clinical trial through the inhibitory tumor test, prolonging life test, immune test, synergy and reducing toxicity test further validate the role of the sesamin. Methods S180- tumor bearing mice were established and randomly divided into model group, cyelophosphamide （CTX） group （40 mg/kg）, sesamin group with different doses （1.0, 0.5, 0.25 g/kg）, the sesamin was administrated daily via gavage for 10 days, tumor in- hibition rates and life extension rates were recorded; S180-tumor bearing mice were established and randomly divided into model group, sesamin group （0.5, 0.25 g/kg）, CTX group （10 mg/kg）, 5-FU group （20 mg/kg）, sesamin groups （0.5, 0.25 g/kg） and CTX （10 mg/kg）, sesamin groups （0.5, 0.25 g/kg） and 5-FU （20 mg/kg）, the different groups of sesamin were administrat- ed daily via gavage for 10 d, tumor inhibition rates were recorded; S180-tumor bearing mice were established and randomly divided into model group, CTX group （40 mg/kg）, sesamin groups （1.0, 0.5, 0.25 g/kg） ＋ CTX, the different groups of sesamin were administrated daily via garage for 10 days, tumor inhibition rates were recorded. Total number of leukocytes in peripher- al blood, total number of cells in peripheral blood and tumor inhibition rates were recorded. Results The tumor inhibited rates were 28.40% and 25.80% in sesamin groups （1.0 g/kg and 0.5 g/kg）. The life extension rates were 32.24%, 24.18% and 17.96% in sesamin groups （1.0, 0.5, 0.25 g/kg）. Both sesamin （0.5, 0.25g/kg） ＋ CTX and sesamin （0.5, 0.25g/kg） ＋ 5-FU had significant inhibitory effects, the tumor inhibited rates （73.77%, 71.92% and 75.41%, 73.15%） were higher than the theoreti- cal additive effect of two drugs＂ combination （71.72%, 68.50% and 74.96%, 76.74%）. The nucleated cells in bone marrow re- bounded significantly in sesamin groups （1.0 g/kg and 0.5 g/kg, P 〈 0.05）. The number of leukocytes in peripheral blood re- bo

关 键 词：芝麻素 抗肿瘤 协同增效 减毒作用 

分 类 号：R962[医药卫生—药理学]