人心肌肌钙蛋白Ⅰ的分离纯化  

Separation and Purification of Human Cardiac Troponin Ⅰ(cTnⅠ)

作　　者：魏景艳[1] 付平平[2] 宋昂[3] 

机构地区：[1]吉林省临床检验中心,长春130021 [2]白求恩医科大学第三临床学院,长春130021 [3]长春市口腔医院,长春130022

出　　处：《中国生物制品学杂志》2000年第4期218-220,共3页Chinese Journal of Biologicals

基　　金：吉林省卫生厅科研经费资助

摘　　要：目的　制备抗人cTnⅠ单克隆抗体 ,以便进一步制备AMI诊断制剂。方法　用兔骨骼肌Tnc(sTnC)制备TnC Sepharose 4B亲和柱 ,直接从心肌组织中分离提纯人cTnⅠ。结果　所提纯的cTnⅠ经SDS PAGE分析已达电泳纯 ,其相对分子质量和氨基酸组成与文献报道相符。结论　为建立AMI早期诊断试剂奠定了基础。Objective To prepare the McAb to cardiac troponin Ⅰ(cTnⅠ) for further preparation of diagnostic kit for acute myocardiac infarction(AMI).Methods TnC-Sepharose 4B affinity chromatography column was prepared with rabbit skeletal muscles troponin C(sInC) and used for the direct purification of cTn Ⅰ from human cardiac muscle tissue.Results SDS-PAGE proved that the cTnⅠ was electrophoretic pure, and the molecular weight and amino acid component of it were consistent with that reported in documents.Conclusion The study lays a foundation of developing the diagnostic kit for early AMI.

关 键 词：心肌肌钙蛋白Ⅰ 亲和层析 急性心肌梗塞 分离 

分 类 号：R542.22[医药卫生—心血管疾病]