Lys-纤溶酶原的制备及其活力稳定性  

Preparation and Stability of Lys-Plasminogen

作　　者：陈维金[1] 孙宏[1] 关锋[1] 张洪超[1] 陈立新[1] 赵立哲[1] 曲爱军 左泉 李武平[1] 刘淑英[1] 梁放[2] 刘兰英[2] 

机构地区：[1]长春生物制品研究所,长春130062 [2]吉林大学,长春130021

出　　处：《中国生物制品学杂志》2000年第4期221-223,共3页Chinese Journal of Biologicals

摘　　要：目的　制备Lys 纤溶酶原并观察其稳定性。方法　人血浆组分Ⅲ经Lys Sepharore 4B和SephadaxG 2 0 0柱层析纯化。结果　得到分子质量为 90 0 0 0的天然纤维蛋白溶酶原 (G/u Hpg) ,经透析得到N 末端为赖氨酸的纤溶酶原 (Lys HPg) ,其活力较Glu HPg更稳定。结论　为合成酰化纤溶酶原—链激酶复合物 (APSAC)奠定基础。Objective To prepare Lys plasminogen and study on the stability of it.Methods The fraction Ⅲ of human plasma was purified by Lys Sepharose 4B affinity chromatography and Sephadex G 200 gel filtration.Results The nature plasminogen(Glu HPg)with a molecular weight of 90000 was prepared by the above mentioned method and further dialyzed,then Lys HPg was obtained.The 2 kinds of HPg were stored at different temperatures for different time,the result showed that Glu HPg was more stable than Lys HPg.Conclusion The study laid a foundation of synthesis of acylating plasminogen streptokinase complex.

关 键 词：人纤溶酶原 层析 稳定性 制备 纯化 溶栓药物 

分 类 号：TQ464.8[化学工程—制药化工] R977.3[医药卫生—药品]