小牛胸腺素的动物实验及临床应用  

Animal Test and Clinical Application of Calf Thymosin

作　　者：熊仁平[1] 周元国[1] 刘苹[1] 侯淦泉[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所分子生物学中心,重庆400042

出　　处：《中国生物制品学杂志》2000年第4期241-244,共4页Chinese Journal of Biologicals

摘　　要：目的　提取胸腺素并检测药物指标 ,观察其临床应用和动物实验效果 ,探讨胸腺素对哮喘豚鼠肺组织Gα表达的影响。方法　采用酸碱沉淀加离心法制备胸腺素 ,经多肽定量、活性鉴定、细菌培养、毒性试验和热原实验 ,临床疗效观察 ,并以卵蛋白雾化吸入致敏法复制哮喘模型 ,Westernblot分析法测定哮喘豚鼠肺组织Gα水平。结果　所制胸腺素符合中国药典标准。临床疗效确切 ,经治疗后 ,哮喘豚鼠肺组织Giα、Gqα水平显著降低 (P <0 0 5 ) ,Gsα无显著变化。结论　采用酸碱沉淀加离心法可制备胸腺素 ,其治疗哮喘动物的机理可能是通过改变肺组织Gα水平来达到。Objective To extract thymosin,detect the pharmaceutical indexes of it,observe the effect of it in clinical application and animal test,and explore the influence of it on the expression of Ga protein in the lung tissue of guinea pig with asthma.Methods Thymosin was prepared by acid base precipitation and centrifugation,and the pharmaceutical indexes of it were detected by quantification of polypeptide,identification of activity,bacterial culture,toxicity test and pyrogen test.The clinical effect of it was observed.Guinea pig model with asthma was established by aerosol inhalation of egg albumin.The Ga protein level in the lung tissue of guinea pig with asthma was analyzed by Western blot.Results The thymosin met the requirement of Chinese Pharmacopeia.The clinical effect of it was definite.The Gia and Gqa levels in the lung tissues of guinea pigs were significantly decreased after being treated with it (p<0 5).However,no significant change of Gsa level was observed.Conclusion The thymosin prepared by acid base precipitation and centrifugation can be applied in clinic.The curative mechanism of it might be changing the Ga protein level in lung tissue.

关 键 词：胸腺素 哮喘 G蛋白 治疗 作用机理 动物实验 

分 类 号：R562.250.5[医药卫生—呼吸系统] R977.6[医药卫生—内科学]