大鼠NPYY2受体基因的克隆及C端肽的表达和纯化  

Cloning of Neuropeptide Y Y2 Receptor Gene and Expression and Purification of Its C-terminal Peptide.

作　　者：程希平[1] 肖华胜[1] 黄文晋[1] 张萍[1] 鞠躬[1] 张旭[1] 

机构地区：[1]西安第四军医大学中国人民解放军神经科学研究所,西安710032

出　　处：《生物化学与生物物理进展》2000年第6期633-636,共4页Progress In Biochemistry and Biophysics

基　　金：国家自然科学基金!(395250 10 ;39830 16 0和 395 0 0 0 45 )资助

摘　　要：Y2受体亚型是早期发现的NPY的两种主要受体亚型之一 ,参与NPY所介导的多种生理和病理功能 .为了制备Y2受体抗体和开展Y2受体的定位研究 ,用RT PCR方法从大鼠海马的总RNA扩增NPY的Y2受体全长基因 ,接着PCR扩增Y2受体的C端片段 ,克隆入表达载体中 ,建立了重组NPYY2受体C端肽的表达菌株 。Y2 receptor, one of the two early recognized major receptor subtypes of neuropeptide Y (NPY) was considered to be involved in multiplicate biological and pathological functions induced by NPY. In order to make antibody against Y2 receptor and study the distribution of Y2 receptor, NPY Y2 receptor gene was amplified by RT PCR from the total RNA of rat hippocampus. Then the C terminal fragment of Y2 receptor was amplified by PCR and cloned into expression vector. The expression vector of C terminal peptide of NPY Y2 receptor was transformed in E.coli , and the product of expression was purified.

关 键 词：Y2受本 神经肽Y 克隆 表达 

分 类 号：Q42[生物学—神经生物学] R322.81[生物学—生理学]