四个鲫鱼品系线粒体DNA的限制性酶切分析  

ANALYSIS ON THE RESTRICTION FRAGMENTS'LENGTH OF THE MITOCHON-DRIAL DNA FROM FOUR STRAINS OF Carassius auratus

作　　者：黎双飞[1] 刘少军[1] 刘筠[1] 张轩杰[1] 罗琛[1] 

机构地区：[1]湖南师范大学生命科学学院,长沙410081

出　　处：《Zoological Research》2000年第6期432-436,共5页动物学研究（英文）

基　　金：湖南省自然科学基金重点资助项目

摘　　要：用差速离心和核酸酶消化法从红鲫 (C auratusredvar .)、湘鲫 [F1hybridsofredcruciancarp (♀ )×commoncarp (♂ ) ]、野鲫 (C auratusauratus)和白鲫 (C auratuscuvieri)的肝组织及白鲫的卵巢中提取和纯化线粒体DNA。用 9种内切酶 (EcoRⅠ、HindⅢ、PstⅠ、BglⅡ、BamHⅠ、XhoⅠ、XbaⅠ、SalⅠ和KpnⅠ )进行单酶酶解 ,经琼脂糖凝胶电泳分析 ,检测出PstⅠ、KpnⅠ和BglⅡ 3种酶在品系间存在限制性片段长度多态性 ,但并未检测出品系内的限制性片段长度多态性。计算出红鲫、湘鲫、白鲫和野鲫的mtDNA大小分别约为 16 19、 16 0 2、 16 6 0和 16 0 6kb。根据限制性酶切片段共享度 ,计算出 4个品系间的遗传距离 ,结果表明存在直接亲缘关系的红鲫与湘鲫之间的遗传差异最小 ,证实了红鲫与子代湘鲫之间mtDNA遵循母系遗传的特性。The mitochondrial DNAs (mtDNAs) from liver of C auratus red var.,F 1 hybrids of red crucian carp (♀)×common carp (♂), C auratus cuvieri and C auratus auratus and ovary of C auratus auratus were isolated and purified by the means of differential centrifugation and nuclease digestion. After having been digested by 9 restriction endonucleases ( Eco RⅠ, Hin dⅢ, Pst Ⅰ, Bgl Ⅱ, Bam HⅠ, Xho Ⅰ, Xba Ⅰ, Sal Ⅰ and Kpn Ⅰ),the mtDNAs were analysed by using agarose gel electrophoresis. The results showed that Pst Ⅰ, Kpn Ⅰ and Bgl Ⅱ were the restriction fragments length polymorphism among four strains,but without the polymorphism within strains. The mitochondrial DNA size was about 16 19?kb in C auratus red var ,16 02?kb in hybrids,16 60?kb in C auratus cuvieri and 16 06?kb in C auratus auratus respectively. According the proportion of shared restriction fragments,the genetic distances between strains were calculated. The results manifested that the genetic distance was the smallest between C auratus red var. and its filial generation hybrids,and that mtDNA followed the character of matrilinear inheritance.

关 键 词：鲫鱼 线粒本DNA 限制性内切酶 遗传距离 

分 类 号：Q78[生物学—分子生物学]