陶瓷化骨的磷酸钙／丹参涂层及对成骨细胞生长影响的研究  

Calcium Phosphate and Danshen Compound Coating on True Bone Ceramic

作　　者：姜红江[1] 谭训香[1] 鞠海洋[1] 宋修刚[1] 仇志烨[2] 

机构地区：[1]山东文登整骨医院,山东文登264400 [2]清华大学材料科学与工程系,北京100084

出　　处：《生物工程前沿（中英文版）》2013年第3期30-36,共7页Biotechnology Frontier

基　　金：受山东省中医药科学技术研究项目资助（2005-2006-83）

摘　　要：本研究探讨陶瓷化骨磷酸钙／丹参涂层后理化及生物学性能的变化，提供理想的骨组织工程支架材料。在研究中，将陶瓷化骨与磷酸钙丹参充分混合、负压抽吸，得到均匀涂层。样本分别进行x线衍射（XRD）分析及x线能谱（EDS）分析、扫描电镜（SEM）观察，了解理化性能变化；与骨髓来源的成骨细胞联合培养观察，检测ALP活性，评价其生物学性能。结果表明，陶瓷化骨表面涂层后成分、Ca／P比无明显变化，保留了天然的三维多孔结构，与成骨细胞联合培养ALP活性明显高于对照组，能促进成骨细胞的增殖和分泌。因此，陶瓷化骨经含生物活性因子或药物的磷酸钙涂层后，增加了生物活性，与成骨细胞复合后是一种理想的骨修复材料。The present work is to investigate the physicochemical and biological properties of true bone ceramic coated with calcium phosphate/Danshen compound, and offer an ideal scaffold material for bone tissue engineering. In the study, surface coated true bone ceramic were obtained by fully mixed with Calcium phosphate/Danshen compound and sucked under negative pressure. Scanning electron microscopy （SEM）, X-ray diffraction （XRD） and energy dispersive spectroscopy （EDS） were employed to study the coated composition. The scaffold was co-cultured with osteoblast derived from bone marrow stromal cells, alkaline phosphatase （ALP） activity at 2, 4, 6 and 8 days of cultivation were measured. The results indicated that there was no obvious change in ingredient and Ca/P ratio of surface coated true bone ceramic, and the natural three-dimensional porous structure was remained. The ALP activity was higher in the surface coated group than in the control group, indicated that the surface coated true bone ceramic significantly promoted cells adhesion, proliferation and secretion. Therefore, bioactivity of true bone ceramic was increased by the surface coating of calcium phosphate/Danshen compound, such coating composition with seed cells is an ideal material for bone repair.

关 键 词：陶瓷化骨 磷酸钙 丹参 表面涂层 成骨细胞 

分 类 号：S162.5[农业科学—农业气象学]