噬菌体表面表达抗汉坦病毒衣壳蛋白单链抗体的研究  

Study on the Phage Display Expressing Anti-Hantavirus Capsid Protein scFv

作　　者：李光富[1] 唐家琪[1] 陈宇萍[2] 王琰[2] 操敏[1] 李先富[1] 潘秀珍[1] 

机构地区：[1]南京军区军事医学研究所,南京210002 [2]海军总医院,北京100037

出　　处：《中国病毒学》2000年第4期409-413,共5页Virologica Sinica

基　　金：国家自然科学基金资助项目(39670654)

摘　　要：The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of scFv is consistent with that of cloned antibody variable region from F3 strain hybridom against HV capsid protein.The expressed scFv was proved to be able to bind HV antigen by ELISA.The expression vector of anti Hantavirus(HV) capsid protein scFv was constructed by DNA recombination and PCR technique,and expressed in E.coli . The expressed scFv was fused with the phage GeneⅢ protein, and located on the surface of phage.The sequencing of scFv demonstrated that the sequence of scFv is consistent with that of cloned antibody variable region from F3 strain hybridom against HV capsid protein.The expressed scFv was proved to be able to bind HV antigen by ELISA.

关 键 词：单链抗体 肾综合征出血热病毒 噬菌体表面表达 

分 类 号：Q939.48[生物学—微生物学]