中华鳖不同组织Sox基因表达的RT—PCR分析  

The RTPCR Expression Analysis on Sox Gene of Different Tissues from the Trionyx Sinensis

作　　者：聂刘旺[1] 单祥年[2] 汪鸣[1] 郭超文[1] 鲁晓萱 

机构地区：[1]安徽师范大学生物系,中国安徽芜湖241000 [2]南京师范大学生物系,中国江苏南京210097

出　　处：《激光生物学报》2000年第3期181-184,共4页Acta Laser Biology Sinica

基　　金：安徽省和江苏省自然科学基金资助项目!( 974 1 2 0 0 2 and 9750 61 1 2 )

摘　　要：本文采用 RT— PCR技术 ,研究了中华鳖不同组织 Sox基因的表达 ,并通过 PCR直接克隆法 ,分析了来自睾丸、脑和脾组织中的 Sox基因序列。结果表明 :在中华鳖的成体组织中 ,Sox基因在脑、心肌、肾、脾和雄性的睾丸组织中均有不同程度的表达 ,而在肌肉、肝脏和雌性的卵巢中则无表达 ,显示该基因具有组织表达特异性。克隆分析显示 ,在睾丸组织中表达的是 TSSox1和 TSSox4基因 ,而在脑组织中表达的是 TSSox2和TSSox4基因 ,脾组织中表达的是 TSSox4基因。此结果表明 Sox基因不仅在性别决定中起作用 ,还可能在神经系统。In the present paper, the Sox gene expression analysis of different tissues from the Trionyx sinensis was studied by using RTPCR, and Sox gene fragments of expression from the testicle, brain and spleen were cloned using RTPCR products. The results show that Sox gene has specific expression in the testicle, brain, spleen, cardiac muscle and kindney and hasn't expression in muscle, liver and ovary. The results of clone reveal that the Sox genes of expression in the testicle are TSSox1 and TSSox 4, and those are TSSox2 and TSSox4 in the brain, and that is TSSox4 in the spleen. This suggests that the Sox gene act important role not only on the sex determination, but also on the development of neural system, immunocyte system and the differentiattion of male germ cell.

关 键 词：中华鳖 RT-PCR 序列分析 基因表达 

分 类 号：Q959.63[生物学—动物学]