胆绿素的激光表面增强拉曼光谱研究(英文)  

Surface-enhanced Raman Spectroscopy of Biliverdin

作　　者：胡军[1] 方清 盛荣生[2] 徐知三[2] 曾云鹗[2] 

机构地区：[1]海南大学生物科学技术研究所,中国海南海口570228 [2]武汉大学化学系,中国湖北武汉430072

出　　处：《激光生物学报》2000年第3期221-227,202,共8页Acta Laser Biology Sinica

摘　　要：本文中 ,SERS被用来测定胆绿素 ,并对溶液中的 p H和 Na Cl浓度对谱图的影响进行了研究。测定下限可达 1 .2 8× 1 0 -7M,且摄谱时间只需 1 5分钟。另外 ,讨论了胆绿素在银胶表面的吸附状态及质子化状态。研究表明胆绿素在银胶表面是呈近乎平面的环状结构吸附 ,其内酰胺环 A上氧原子和吡咯环上的氮原子可能发生了质子化。In this paper, we obtained an excellent SERS spectrum of biliverdin. The effect of pH, ion concentration on the SERS spectrum of biliverdin and the orientation of biliverdin on the surface of silver colloid was discussed. In the case, the biliverdin might lie flat on the surface of silver collide. The detect limit of biliverdin with SERS technique was down to 1.28 ×10 -7 M by a rapid scan(about 15min). The experimental results indicated that the biliverdin might adopt a near planar and cyclic conformation on the surface of silver colloid particle. The rings of biliverdin were flat on the surface of silver colloid particle with the N atom of pyrrole ring C and O atom of lactam ring A protonated.

关 键 词：胆绿素 激光表面增强拉曼光谱 表面吸附取向 

分 类 号：O657.37[理学—分析化学] Q586.03[理学—化学]