高糖高脂肪酸环境下维生素D对分化后前脂肪细胞脂肪合成的影响  

Influence of vitamin D on fat synthesis of preadipocyte after differentiation under the environment of high glucose and high free fatty acids

作　　者：张力翔[1] 缪珩[1] 

机构地区：[1]南京医科大学第二附属医院,南京210011

出　　处：《山东医药》2013年第35期21-23,I0003,共4页Shandong Medical Journal

基　　金：南京医科大学科技发展基金项目(2010NJMUZ50)

摘　　要：目的探讨在高糖、高脂肪酸环境下维生素D的活性形式1,25(OH)2D3(VD3)对分化后前脂肪细胞脂肪合成的影响。方法将前脂肪细胞3T3-L1细胞分为对照组(C组)、高糖组(G组)和高糖高脂肪酸组(GF组)后进行诱导分化,观察加入VD3后最终成熟脂肪细胞中脂肪含量的改变,采用油红O染色和异丙醇萃取法半定量检测细胞内脂肪含量;用RT-PCR法检测各组细胞维生素D受体(VDR)、固醇调节元件结合蛋白-1c(SREBP-1c)和激素敏感脂肪酶(HSL)mRNA的表达水平。结果与C组比较,G组和GF组脂肪细胞内脂肪含量明显增高(P<0.05),VD3可减少C组脂肪含量(P<0.05),但是对G组和GF组脂肪含量无影响(P>0.05)。与C组比较,G组和GF组VDR mRNA表达降低(P<0.05),VD3可提高三组细胞VDR mRNA的表达(P<0.05)。与C组比较,G组和GF组的SREBP-1c、HSL mRNA表达升高(P<0.05);VD3减少C组SREBP-1c mRNA水平,对G组和GF组的SREBP-1c、HSL以及C组的HSL mRNA无影响(P>0.05)。结论在高糖、高脂肪酸的环境下,VD3已失去对前脂肪细胞脂肪合成的抑制作用。Objective To investigate the influence of 1,25 （OH） 2 D3 （ VD3 ） which is the active form of vitamin D on fat synthesis of preadipoeytes after differentiation under the environment of high glucose and high free fatty acids （FFAs）. Methods 3T3-L1 preadipocytes were divided into 3 groups： the control group （group C）, high glucose group （group G） and high glucose with high free fatty acids group （ group GF） , and then they were induced to differentiation into mature fat cells with or without adding VD3 ; oil red staining was employed to detect the total amount of fat in different groups ; RT- PCR was used to detect the mRNA levels of vitamin D receptor（ VDR）, sterol regulatory element binding transcription fac- tor 1 （ SREBP-1 c） and hormone sensitive lipase （HSL）. Results Compared with group C, the fat amount was significantly increased in G and GF groups （ P 〈 0.05 ）, VD3 decreased the fat amount in group C but had no effect on that of G and GF groups （P 〉 0.05 ） ; the VDR mRNA level was decreased in G and GF groups, and VD3 could increase the VDR mRNA level in three groups （P 〈 0.05 ）; the SREBP-lc and HSL mRNA levels were up-regulated in G and GF groups （P 〈 0.05） ; VD3 decreased the SREBP-lc mRNA level in group C, but had no effect on SREBP-lc and HSL mRNA in G and GF groups as well as HSL mRNA in group C （ P 〉 0.05 ）. Conclusion VD3 had lost the ability to inhibit the fat synthesis in 3T3-L1 cells when exposed under the high glucose and high free fatty acids environment.

关 键 词：维生素D 维生素D受体 3T3-L1 肥胖 糖尿病 

分 类 号：R589[医药卫生—内分泌]