基于苯硼酸-糖蛋白相互作用的过氧化氢生物传感器  

Construction of hydrogen peroxide biosensor based on the interaction between phenylboronic acid and glycosylated protein

作　　者：张立[1] 李雅华[1] 梁汝萍[1] 邱建丁[1] 

机构地区：[1]南昌大学化学系,江西南昌330031

出　　处：《南昌大学学报（理科版）》2013年第4期359-365,共7页Journal of Nanchang University(Natural Science)

基　　金：国家自然科学基金资助项目(21265012;21265017);教育部新世纪优秀人才支持计划资助项目(NCET-11-1002)

摘　　要：利用4,4'-二硫代二丁酸(DTBA)和3-氨基苯硼酸(PBA)反应合成了含苯硼酸基的二硫化物DTBAPBA,通过自组装技术将其固定于金电极表面。利用苯硼酸分子在水溶液中较温和以及易控制的条件下即可与1,2/1,3-邻二醇反应形成五元或六元环化合物的特点,将糖基化蛋白辣根过氧化物酶(HRP)共价键合于DTBAPBA修饰电极表面,构建了用于H2O2检测的生物传感界面。采用循环伏安、计时电流等方法对传感界面的构建过程和传感器的性能进行了研究。结果表明,在8.3×10-6-5.3×10-2 mol·L-1范围内,传感器响应电流与H2O2浓度呈良好的线性关系,可用于H2O2的灵敏性和选择性检测。Disulfide compound DTBA--PBA that carried phenylboronic acid groups at its ends was prepared by coupling of 3-aminophenylboronic acid （PBA） with 4,4′-Dithiodibutyric acid （DTBA） ,and it was fastened to gold electrode surface through self-assembly technique. Phenylboronic acid could react with 1,2 - or 1,3-diols forming five- or six-membered cyclic complexes in solution under mild and easily controllable conditions. Aware of this nature,horseradish peroxidase （HRP） which has a high degree of glycosylation of about 16.8%-21% ,covalently bonded to the gold electrode surface for H2O2 detection. The performance of the biosensor was characterized by cyclic voltammetry and the chronoamperometry. The results showed that the linear response of the HRP modified electrode to H2O2 was in the concentration range of 8.3×10^-6- 5.3×10^-2 M with a detective limit of 2.24 μM （S/N=3） ,the equation of linear regression was Ip （μA）=18.89C[H2O2]（μM）＋0. 084 15 with a linear correlation coefficient of 0. 9992 .

关 键 词：苯硼酸 自组装 辣根过氧化物酶 生物传感 

分 类 号：O657.1[理学—分析化学]