新城疫病毒广东基因Ⅶ型分离株的分离鉴定及F基因克隆与序列分析  

Identification and Sequence Analysis of F Gene of GenotypeⅦ Newcastle Disease Virus Guangdong Isolated

作　　者：刘佳佳[1] 严专强[1] 刘迪[2] 李欣莲[2] 覃健萍[2] 谢青梅[1] 毕英佐[1] 薛春宜[3] 陈峰[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]广东温氏食品集团股份有限公司,广东云浮527439 [3]中山大学生命科学学院,广东广州510006

出　　处：《动物医学进展》2013年第10期50-53,共4页Progress In Veterinary Medicine

基　　金：广东省产学研资助项目(2010B090301019)

摘　　要：2011年从广东一疑似新城疫病毒感染的养禽场通过SPF鸡胚接种试验、HA及HI试验和动物回归试验,生物学毒力指标(MDT,ICPI)测定,分离到一株新城疫毒株,命名为GD-X1-2011,并对该毒株进行了F基因的序列测定分析。GD-X1-2011株的MDT为45h<60h,ICPI为1.88>1.6,分离毒株具有血凝性且能被NDV La Sota标准阳性血清抑制,动物回归试验鸡出现典型的新城疫症状。毒株GD-X1-2011F基因的裂解位点序列为112R-R-Q-K-R-F117,属于强毒裂解序列,其F基因与基因Ⅶ型毒株SDWF07-2011同源性为97.5%,与其他不同基因型毒株的同源性在83.6%～90.1%之间。进化树分析GDX1-2011株属于基因Ⅶ型毒株。One Newcastle disease virus strain was isolated from Guangdong Province in 2011. Throgh infecting 10-day old chick embryos, HA, HI and animal experiments,the strain was named as GD-X1-2011. The full sequence of the F gene of this strain was cloned and sequenced. The biological virulence of GD-X1-2011 was MDT〈45 h and ICPI〉I. 6, the virus has HA activity and can be neutralized by the positive serum of ND La Sota. The chicken appeared the classic symptom through animal experiments. The cleavage site sequence of GD-X1-2011 strain was 112R-R-Q-K-R-F117. The F gene of GD-X1-2011 virus showed 97.5% homology with the genotype Ⅶ virus SDWF07-2011, and 83.6%-90.1% with the other gene type viruses. Phylogenetic analysis of F gene showed that the GD-X1-2011 strain was classified into genotype Ⅶ. So the NDV prevalent strain in South China is the gene Ⅶ type.

关 键 词：新城疫病毒 分离鉴定 F基因 序列分析 

分 类 号：S852.659.5[农业科学—基础兽医学] Q785[农业科学—兽医学]