丙泊酚对呼吸机所致肺损伤大鼠水通道蛋白5表达的影响  

The effects of propofol on the ventilator-induced lung injury and aquaporins 5 expression in rats

作　　者：陈弘 高巨 林舜艳 黄涛 周罗晶 

机构地区：[1]扬州大学临床医学院江苏省苏北人民医院麻醉科,江苏扬州225001 [2]扬州大学临床医学院江苏省苏北人民医院科教处,江苏扬州225001

出　　处：《广东医学》2013年第18期2761-2763,共3页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81171838)

摘　　要：目的评价丙泊酚对呼吸机所致急性肺损伤和水通道蛋白5(AQP-5)表达的影响。方法健康雄性Wistar大鼠50只,采用随机数字表法,将其随机分为5组,每组10只:常规潮气量通气组(A组,V T=8 mL/kg)、大潮气量通气组(B组,V T=30 mL/kg)、丙泊酚不同时点给药组(C^E组,V T=30 mL/kg)。C组、D组和E组分别于给予大潮气量通气前1 h、给予大潮气量通气即刻和给予大潮气量通气后1 h时静脉注射丙泊酚5 mg/kg,随后以10 mg/(kg·h)速率输注至机械通气4 h结束。测定肺泡灌洗液蛋白、肺通透指数(LPI)及肺组织湿/干重量比(W/D);HE染色光镜下观察肺损伤程度;采用Western blot和RT-PCR法分别测定肺组织AQP-5的蛋白及mRNA表达。结果与A组比较,B^E组W/D、LPI增加,AQP-5表达下调(P<0.05);与B组比较,C组、D组W/D、LPI下降,AQP-5表达上调(P<0.05),HE染色肺损伤程度减轻。结论丙泊酚早期给药能减轻呼吸机所致肺损伤,其保护机制与上调肺组织AQP-5的表达有关。Objective To investigate the effects of propofol on ventilator - induced lung injury and the expression of aqnaporin 5 （ AQP - 5 ） in rats. Methods Fifty male Wistar rats were randomly divided into 5 groups ： standard venti- lation group （ Group A, VT = 8 mL/kg） ; high - tidal volume ventilation group （ Group B, Vr = 30 mL/kg） ; propofol trea- ted groups （ Group C, D, and E, in which propofol 5 mg/kg 1 h before, immediately after and 1 h after high - tidal vol- ume ventilation （30 mL/kg） was given, respectively, followed with infusion of propofol at 10 mg/（ kg ~ h） until 4 h after ventilation）. Lung tissues were collected for calculation of wet/dry lung weight ratio （W/D）, lung permeability index （LPI）, and lung pathologic change. The expressions of AQP- 5 mRNA and protein were also assessed. Results When compared with Group A, significant increase in W/D and LPI, with down - regulation in AQP - 5 mRNA and protein were observed in the other 4 groups （ P 〈 0. 05 ）. When compared with Group B, significantly lower W/D and LPI, with up - regulation in AQP - 5 were observed in Group C and D （ P 〈 0. 05 ）. Morphological changes and neutrophil sequestration were also less in Group C and D. Conclusion Early propofol administration provides protective effects on the lung injury induced by ventilator, which may be related to the up - regulation of AQP - 5.

关 键 词：丙泊酚 呼吸 人工 呼吸窘迫综合征 成人 水通道蛋白5 

分 类 号：R563[医药卫生—呼吸系统]