13个大果油茶品种遗传与亲缘关系的ISSR分析  

ISSR analysis on genetic relation among 13 species of Camellia crepnelliana Tutch.

作　　者：杨翠芳[1,2,3] 陈伯伦[1] 黄诚梅[1,2] 吕维莉[1,2] 

机构地区：[1]广西农业科学院,南宁530007 [2]广西作物遗传改良生物技术重点开放实验室,南宁530007 [3]广西师范大学,广西桂林541004

出　　处：《南方农业学报》2013年第9期1421-1425,共5页Journal of Southern Agriculture

基　　金：广西自然科学基金项目(2010GXNSFA0138081)

摘　　要：[目的]探讨广西大果油茶资源的遗传多样性及其亲缘关系,为其种质资源利用和品种选育等提供参考.[方法]采用ISSR分子标记技术对13个大果油茶品种（系）的遗传与亲缘关系进行分析.[结果]从99条ISSR引物中筛选出6条多态性引物,共扩增出56条带,其中54条为多态性带,多态性比例为95.2％,平均每个引物扩增的条带数为9.33条.供试大果油茶品种间的遗传相似系数范围为0.2222～0.7826,平均相对遗传相似系数为0.5293.UPGMA法聚类分析结果表明,供试的13个大果油茶品种（系）可分为3个类群.UPGMA法聚类分析结果表明供试的13个大果油茶品种（系）可分为3个类群.[结论]13个广西大果油茶品种资源间的遗传基础较宽.[ Objective ]The paper focused on assess the genetic variability and diversity of Camellia crepnelliana Tutch. to provide referenus for resource utilization and eultivar breeding. [ Method ]Genetic relation among 13 species of Camellia crepnelliana Tutch. was analyzed by inter-simple sequence repeat （ISSR） makers. [Resuh]A total of 56 bands were de- tected using 6 ISSR primers, of which 54 were polymorphic, and the total percentage of polymorphic bands was 95.2%. The dissimilarity coefficient of the 13 Camellia crepnelliana Tutch. varieties ranged from 0.2222 to 0.7826 using the Nei and Li （1913） statistic method, with an average of 0.5293. Total of 13 Camellia crepnelliana Tutch. varieties were divided into 3 groups by UPGMA according to the average value of similarity coefficient. [Conclusion]The genetic base of 13 Camellia crepnelliana Tuteh. varieties resources is wide.

关 键 词：大果油茶 ISSR分子标记 遗传多样性 聚类分析 广西 

分 类 号：S794.401[农业科学—林木遗传育种]