HPLC-ELSD法同时测定四逆散不同配伍中柴胡皂苷a、b_2、c的含量  

Simultaneous Determination of Saikosaponins a,b_2 and c in Different Component Combinations of Sini Powders by HPLC-ELSD

作　　者：杨丽娜[1] 温静[1] 梁佳佳[1] 郑卫华[1] 张丽丽[1] 鲁亚楠[1] 熊志立[1] 

机构地区：[1]沈阳药科大学药学院,沈阳110016

出　　处：《中国药师》2013年第9期1275-1278,共4页China Pharmacist

基　　金：国家自然科学基金项目(编号:81202786)

摘　　要：目的:建立高效液相色谱-蒸发光散射检测(HPLC-ELSD)法同时测定四逆散不同配伍中柴胡皂苷a,b_2,c的含量。方法:采用Hypersil BDS C_(18)色谱柱(250 mm×4.6 mm,5μm),以乙腈-水为流动相进行梯度洗脱;流速:1.0 ml·min^(-1),ELSD检测器,漂移管温度:100℃,气体:空气,气体流速:2.0 L·min^(-1),柱温:室温。结果:柴胡皂苷a,b_2,c分别在7.74~129.00,7.86~131.00,5.12~85.30μg的范围内线性关系良好(r分别为0.999 2,0.999 2,0.999 3);平均回收率分别为98.6%,99.3%,100.7%,RSD分别为2.5%,1.8%,2.7%(n=6)。结论:白芍中的成分可降低柴胡皂苷a、c的煎出量;枳实中的成分可提高柴胡皂苷a、b_2、c的煎出量;与单味柴胡药材相比,配伍组合中柴胡皂苷b_2的含量均显著增加。Objective ： To develop an HPLC-ELSD method for the simultaneous determination of saikosaponins a, bE and c in dif- ferent component combinations of Sini powders. Method： The analysis was performed on a Hypelil BDS Cts column （250 mm x 4.6 ram, 5 μm） at the room temperature with gradient elution of acetoritrile-water as the mobile phase and using ELSD as the detector. The drift tube temperature was 100℃ , and the gas flow rate was 2.0 L .min-j. Result： Saikosaponins a, b2 and c was linear o ver the range of 7.74-129.00, 7.86-131. 00 and 5.12-85.30μg with r of 0. 999 2, 0. 999 2 and 0.999 3, respectively. The average recovery was 98.6% （RSD = 2.5 % , n = 6 ） , 99.3 % （RSD = 1.8 %, n = 6 ） and 100.7 % （RSD = 2.7 %, n = 6 ） for Saikosaponins a, b2 and c, respectively. Conclusion： The composition in Paeonia lactiflora Pall. can decrease the decocting quantity of saikosaponins a and c. The composition in Citrus aurantium L. can increase the decocting quantity of saikosaponins a, b2 and c. Compared with the single Bupleurum herbs, the decocting quantity of saikosaponin b2 is significantly increased in different component combinations of Sini oowders.

关 键 词：四逆散 配伍研究 柴胡皂苷A 柴胡皂苷b2 柴胡皂苷c 高效液相色谱-蒸发光散射检测法 

分 类 号：R927.2[医药卫生—药学]