HPLC法测定川贝枇杷糖浆中齐墩果酸的含量  

Determination of Oleanolic Acid in Chuanbei Pipa Syrups by HPLC

作　　者：刘峰 

机构地区：[1]日照市药品检验所,山东日照276800

出　　处：《中国药师》2013年第9期1371-1373,共3页China Pharmacist

摘　　要：目的:建立HPLC法测定川贝枇杷糖浆中齐墩果酸的含量。方法:色谱柱为Hypersil C_(18)柱(250 nn×4.6 mm,5μm),流动相为乙腈-水(95∶5),流速为1 rr·min^(-1),检测波长为210 nm,柱温为30℃。结果:齐墩果酸的进样量在0.18~3.60μg范围内,峰面积与进样量之间线性关系良好,r=0.999 5,平均回收率为96.5%(RSD=0.91%,n=6)。结论:本方法准确可靠,专属性好,可用于测定川贝枇杷糖浆中齐墩果酸的含量。Objective ： To develop an HPLC method for the determination of oleanolic acid in Chuanbei Pipa syrups. Method： The separation was performed on a Hypersil C18 column（250 mmx 4.6 mm ,5 p.m） , the mobile phase consisted of acetonitrile-water （95： 5） at the flow rate of 1 ml · min-1 , the detection wavelength was set at 210 nm , and the column temperature was Result： There was a good linearity between the peak areas and the quantity of oleanolic acid within the range of 0.18-3.60μg（ r = 0.999 5 ）. The average recovery was 96.5% （RSD = 0.91% , n = 6 ） . Conclusion： The established method is sensitive, reliable and specific, and can be used in the determination of oleanolic acid in Chuanbei Pipa syrups.

关 键 词：川贝枇杷糖浆 齐墩果酸 高效液相色谱法 含量测定 

分 类 号：R927.2[医药卫生—药学]