丝胶对2型糖尿病大鼠肝糖原含量的影响  

EFFECTS OF SERICINE ON LIVER GLUCOGEN CONTENT IN TYPE 2 DIABETES MELLITUS RATS MODEL

作　　者：张艳[1] 王福[1] 乔志燕[2] 梅立新[1] 温海玲[2] 陈志宏[1] 

机构地区：[1]承德医学院,河北承德067000 [2]承德医学院附属医院

出　　处：《承德医学院学报》2013年第5期365-367,共3页Journal of Chengde Medical University

基　　金：河北省自然科学基金项目(H2012406018;H2013406096)

摘　　要：目的:观察丝胶对2型糖尿病大鼠血糖及肝糖原含量的影响。方法:60只雄性SD大鼠随机分为5组,正常对照组、糖尿病模型组、丝胶治疗组、阳性对照组、丝胶预防组,每组12只大鼠。链脲佐菌素(STZ)连续腹腔注射制作2型糖尿病大鼠模型。待模型成功建立后,模型组大鼠不做任何处理;丝胶治疗组大鼠给予丝胶(2.4g/kg/d)灌胃,阳性对照组大鼠给予二甲双胍(55.33mg/kg/d)灌胃;丝胶预防组大鼠于注射STZ前给予丝胶(2.4g/kg/d)灌胃。采用葡萄糖氧化酶法检测各组大鼠的空腹血糖,过碘酸-Schiff染色法观察各组大鼠肝糖原的含量。结果:与糖尿病模型组比较,丝胶治疗组、阳性对照组、丝胶预防组大鼠的空腹血糖显著降低(P<0.01),肝糖原的含量明显增高(P<0.01);且丝胶治疗组、丝胶预防组与阳性对照组比较无明显差别(P>0.05)。结论:丝胶可通过增加2型糖尿病大鼠肝糖原的合成降低空腹血糖,发挥对肝糖原储备的保护作用。Objective： To observe the effects of sericine on blood glucose and liver glucogen in type 2 diabetes mellitus rats model. Methods：60 male SD mrs were randomly divided into 5 groups （n=12）： normal control group, diabetes model group, sericine treatment group, positive eontrol group and sericine prevention group. The type 2diabetes model rats were induced by continuous intraperitoneal injection of streptozotocin （STZ）. After successfully establishing the diabetes animal model, the rats in diabetes model group were not given any more treatment, the rats in sericine treatment group were lavaged with sericine （2.4g/kg/d）, the rats in positive control group were lavaged with metformin （55.33mg/kg/d）. The rats in sericine prevention group were lavaged with sericine （2.4g/kg/d） before injecting STZ. Glucose oxidase was used to detect the fast blood glucose of rats in each group, PAS to detect the liver glucogen content. Results：Compared with rats in model group, the blood glucose of rats in sericine treatrnent group, positive control group and sericine prevention group significantly decreased （P〈 0.01）; while the liver glucogen content increased obviously （P〈 0.01）. Moreover, there had no obvious differences between sericine treatment group, sericine prevention group and positive control group （P〉 0.05）. Conclusions： Sericine can significantly reduce the fast blood glucose of type 2 diabetes mellitus rats by increasing synthesis of liver glycogen, so to protect liver glycogen storage.

关 键 词：丝胶 肝糖原 2型糖尿病 

分 类 号：R587.1[医药卫生—内分泌]