好氧/厌氧条件下混合电子供体对非光合微生物菌群固碳效率影响的析因实验分析  

Effect evaluation of different electron donors on CO_2 fixation by a non-photosynthetic microbial community under aerobic or anaerobic conditions using factorial design

作　　者：胡煜[1] 胡佳俊[1] 侯星恒[2] 苏保兴 孙佩哲[1] 王磊[1] 

机构地区：[1]同济大学环境科学与工程学院污染控制与资源化研究国家重点实验室,上海200092 [2]中国华电集团公司上海分公司,上海200011 [3]上海奉贤燃机发电有限公司,上海200238

出　　处：《工业微生物》2013年第5期1-7,共7页Industrial Microbiology

基　　金：国家自然科学基金项目(21177093;21307093);国家863课题(2012AA050101);中国博士后科学基金(2013M531220);中央高校基本科研业务费专项基金(0400219191)

摘　　要：CO_2是主要的温室气体之一,但同时也是地球上最丰富的碳源,因此回收利用CO_2具有重要意义。本研究通过混合电子供体析因实验,定量描述了好氧/厌氧条件下Na_2S、NaNO_2、Na_2S_2O_23种电子供体的用量和比例与非光合微生物茵群固碳效率之间的关系,并分析了3种电子供体之间的两两交互作用,比较了两种条件下固碳效率。结果表明,电子供体析因实验的回归模型与实验值有较好的拟合度,能对不同配比混合电子供体的固碳效率进行较好地预测。电子供体交互作用分析结果表明,好氧条件下,NaNO_2与Na_2S对茵群固碳能力有互相抑制的作用,Na_2S与Na_2S_2O_3则具有相互促进的作用,NaNO_2单独作用时,对微生物茵群的固碳作用有较大的促进作用,而Na_2S_2O_3的加入则产生了一定的抑制效应。厌氧条件下,Na_2S单独作用能对茵群固碳效率起到促进作用,NaNO_2或Na_2S_2O_3的加入能对Na_2S的促进作用产生抑制,NaNO_2与Na_2S_2O_3能产生相互促进作用。厌氧条件下的微生物菌群固碳效率明显比好氧条件下的固碳效率低。Carbon dioxide is one of the main greenhouse gases, but it is also the most abundant carbon source, so that its recycle and reuse has become an important issue. In our experiments, a factorial design was used to demonstrate the interaction effects of the quantity and ratio of the three different electron donors （Na2S, NANO2, Na2S2O3） on CO2 fixation quantitatively by a non-photosynthetic microbial community under aerobic or anaerobic conditions. In addition, the inter- actions of the three electron donors were also analyzed pairwise and the CO2 fixation efficiency of two different conditions were also compared. The results showed that the factorial design models were of high quality to predict the CO2 fixation efficiency of the non-photosynthetic microbial community. Under aerobic condition, Na2S and Na2S2O3, each could enhance its CO2 fixation efficiency, and the positive effect was reinforced when both were present. NaNO2as added electron donor could significantly enhance CO2 fixation, but this effect was competitively inhibited when Na2S203 was added to the medium. Under anaerobic condition, Na2S played the dominant role on the enhancement of CO2 fixation rate, but this effect was competitively inhibited when Na2S2O3 or NaNO2 was added to the medium. Under anaerobic conditions, the efficiency of CO2 fixation by non-photosynthetic microbial community was obviously lower than that under aerobic conditions.

关 键 词：非光合固碳微生物 固碳 电子供体 析因实验 

分 类 号：Q93[生物学—微生物学]