利用RAPD法为中药材标准物质建立DNA图谱  

Establishment of DNA polymorphism graphs for standard Chinese medical herbs by RAPD

作　　者：袁天翊[1,2] 阎雨[1,2] 方莲花[1,2] 龚宁波[3] 吕扬[3] 杜冠华[1,2] 

机构地区：[1]中国医学科学院北京协和医学院药物研究所 药物靶点研究与新药筛选北京市重点实验室,北京100050 [2]中国医学科学院北京协和医学院药物研究所天然药物活性物质与功能国家重点实验室,北京100050 [3]中国医学科学院北京协和医学院药物研究所晶型药物研究北京市重点实验室,北京100050

出　　处：《中国新药杂志》2013年第19期2250-2255,共6页Chinese Journal of New Drugs

基　　金：国家“重大新药创制”科技重大专项(2013ZX09103-001-008);科技部科技基础性工作专项重点项目(2007FY130100)

摘　　要：目的:建立中药材标准物质DNA图谱,为该药材鉴定提供参考和实验依据。方法:选取25种随机引物,利用随机扩增多态性DNA技术(Random amplified polymorphic DNA,RAPD)对提取出的中药材DNA进行PCR扩增,得到的产物进行琼脂糖凝胶电泳,并对特异性条带进行统计分析。结果:对68种中药材标准物质进行了考察,对其中8种常用于心血管保护的中药材进行了RAPD法DNA图谱的建立,寻找出它们的特异扩增产物。结论:RAPD法可以用来对中药材进行生物学鉴定,所建立的DNA图谱可用来为之后该种药材的鉴定提供依据。Objective ： To establish DNA graphs for Chinese medical herbs for providing criterion and refer- ence to identify specific Chinese medical materials. Methods： DNA was extracted from crude medicines, and then random amplification polymorphic DNA （RAPD） method was used to separately amplify genome DNA by 25 single arbitrary primers. The polymorphism PCR products were useful as genetic markers that were separated by agarose gel electrophoresis and detected by staining in gel-stain. Quantity One software was used to analyze the results. Re- sults： This experiment was conducted on 68 standard Chinese medical herbs. Among them DNA polymorphism graphs were set up for 8 medical herbs, and their specific RAPD PCR products were found. Conclusion： RAPD can be used as an effective method to identify Chinese medical herbs. The DNA graphs can be used as reference for these herbs in the future.

关 键 词：中药材 标准物质 DNA图谱 随机扩增多态性DNA 

分 类 号：R282[医药卫生—中药学]