艾炷灸在干预兔动脉粥样硬化形成过程中对主动脉内皮细胞PPARγ及NF-κB的影响  

Effects of moxa cone moxibustion on the expression of PPARγ and NF-κB in aortic endothelial cells of atherosclerotic rabbits

作　　者：刘密[1] 兰蕾[2] 常小荣[1] 郁保生[1] 岳增辉[1] 谢辉[2] 景向红[3] 

机构地区：[1]湖南中医药大学,长沙410007 [2]郴州市第一人民医院,郴州423000 [3]中国中医科学院针灸研究所,北京100700

出　　处：《中华中医药杂志》2013年第10期3032-3036,共5页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家重点基础研究发展计划(973计划)(No.2009CB522904);国家自然科学基金课题(No.30973802;No.30873308);湖南省科技计划项目(No.2012SK3143;No.2013SK3090);中国中医科学院自主选题开放课题(No.ZZKF06008);湖南省中医药科研计划课题(No.2012121);湖南中医药大学青年科研基金项目~~

摘　　要：目的:观察艾炷灸对动脉粥样硬化(AS)兔主动脉内皮细胞过氧化酶体增殖物激活型受体γ(PPARγ)和核因子-κB(NF-κB)的影响,探讨艾炷灸延迟脉粥样硬化斑块(ASP)形成的作用机制。方法:将30只新西兰大耳白兔随机分为3组,每组10只,即空白组,模型组,艾炷灸组。通过高脂饲料喂养及免疫损伤法建立兔AS模型。采用逆转录聚合酶链反应(RT-PCR)分别检测兔主动脉内皮细胞PPARγ、NF-κB mRNA。结果:与空白组比较,模型组PPARγ/GAPDH值明显降低(P<0.01)、NF-κBp65/GAPDH值明显升高(P<0.01);与模型组比较,艾炷灸组PPARγ/GAPDH值增高(P<0.05)、NF-κB/GAPDH值降低(P<0.05)。结论:艾炷灸能通过激活AS兔主动脉内皮细胞PPARγ,抑制NF-κB,阻断血管受损后的炎性反应而保护血管壁,最终起到延迟ASP形成的作用。Objective： To obselve the effects of moxa cone moxibustion on the expressions of peroxisome proliferatoractivated receptor y （PPART） and nuclear factor-kappa B （NF-κB） in aortic endothelial cells of atherosclerosis （AS） rabbits and explore the mechanism of moxa cone moxibustion in delaying the formation of atheromatous plaque （ASP）. Methods： 30 New Zealand white rabbits were randomly divided into 3 groups. Blank group （control group）, model group （AS model group）, moxa cone moxibustion group （AS model combined with direct moxibustion）. AS rabbits models were established by using immunologic injury method combined with feeding high fat diet. The mRNA of PPAR7 and NF-κB in aortic endothelial cells were measured by Reverse Transcription-Polymerase Chain Reaction （RT-PCR）. Results： Compared with the blank group, PPARy/ GAPDH decreased and NF-κBp65/GAPDH increased in model group （P〈0.01）. PPARy/GAPDH in moxa cone moxibustion group was significantly higher while NF-κBp65/GAPDH lower than model group （P〈0.05）. Conclusion： Moxa cone moxibustion can activate the expression of PPARy in aortic endothelial cells of AS rabbits as well as restrain the expression of NF-κB, it can protect vascular wall by blocking blood vessel inflammation after damage, so as to delay the formation of ASP.

关 键 词：艾炷灸 动脉粥样硬化 动脉粥样硬化斑块 过氧化酶体增殖物激活型受体γ 核因子-ΚB 

分 类 号：R245.8[医药卫生—针灸推拿学]