小青龙汤对肺源性心脏病大鼠ANG-Ⅱ、NO及ET-1的影响  

Effects of Xiaoqinglong Decoction on ANG-II, NO and ET-1 of cor pulmonale rats

作　　者：王浩[1] 李泽庚[1] 彭波[2] 王传博[2] 杨程[2] 童佳兵[2] 

机构地区：[1]安徽中医药大学,合肥230038 [2]安徽中医药大学第一附属医院,合肥230031

出　　处：《中华中医药杂志》2013年第10期3052-3054,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家青年自然科学基金项目(No.81001520)~~

摘　　要：目的:观察小青龙汤对肺源性心脏病大鼠血管紧张素Ⅱ(Ang-Ⅱ)、内皮素-1(ET-1)及一氧化氮(NO)含量的变化,了解肺动脉高压与Ang-Ⅱ和ET-1、NO相互关系,进一步探讨小青龙对支饮型(肺源性心脏病)大鼠肺动脉高压的影响。方法:Wistar大鼠60只,每组20只,随机分为正常组、模型组和给药组;参照相关内容拟定大鼠肺源性心脏病中医症候观察表,分别观察5周造模期及2周给药期中医证候的变化。运用多导生理记录仪记录大鼠肺动脉压力,并利用酶联免疫吸附测定法(ELISA)测定Ang-Ⅱ和ET-1、NO的含量。结果:与正常组比较,模型组与给药组均有不同程度的咳嗽、气促、嘴唇紫绀,但给药组症状较模型组显著改善。与模型组比较给药组Ang-Ⅱ和ET-1、NO含量有显著差异(P<0.05,P<0.01)。结论:小青龙汤通过调节Ang-Ⅱ和NO、ET-1含量的动态平衡可以有效的改善大鼠的肺动脉高压。Objective： Observation of Xiaoqinglong Decoction on angiotensin Ⅱ （Ang- Ⅱ ）, endothelin 1 （ET-1） and nitric oxide （NO） content changes in cot pulmonale rats, to understand the mutual relationship between the pulmonary hypertension and Ang- Ⅱ, ET-1, NO, to further discuss the the influence of Xiaoqinglong on the thoracic fluid retention （cot pulmonale） pulmonary hypertension in rats. Methods： 60 Wistar rats, 20 in each group, were randomly divided into three grouop, including normal group, model group and treatment group. The observation form obout rats cot pulmonale in TCM syndrome was drawn up with reference of relevant content, to respectively observe the TCM syndrome changes of 5 weeks model stage and 2 weeks gastric lavage stage. The rats pulmonary artery pressure was recorded by physiological recorder, and the content of Ang-Ⅱ and ET-1, and NO was determine by enzyme-linked immunosorbent assay （ELISA）. Results： Compared with normal group, model group and treatment group were with varying degrees of cough, shortness of breath, purple lips defect, but the symptom in treatment group was obviously improved than the model group. And compared with the medicine group, the content in Ang-Ⅱ, ET-1 and NO was obvious different （P〈0.05）, and in the treatment group, ET-1-Ⅱ changed more obvious than Ang-Ⅱ （P〈0.01）. Conclusion： Xiaoqinglong Decoction could effectively improve the pulmonary hypertension in rats by adjusting the dynamic balance of content in Ang-Ⅱ and NO, ET-1.

关 键 词：小青龙汤 肺源性心脏病 血管紧张素Ⅱ 一氧化氮 内皮素-1 肺动脉高压 

分 类 号：R285.5[医药卫生—中药学]