基于Box-Behnken设计优选南方红豆杉多糖微波提取工艺研究  

Study on Microwave Extraction of Polysaccharides from Branches of Taxus mairei (Lemee et Levl.) S.Y.Hu ex Liu by Response Surface Method

作　　者：李石清 徐磊[2] 熊耀康[2] 张春椿[2] 

机构地区：[1]浙江省食品药品检验研究院,浙江杭州31000 [2]浙江中医药大学药学院,浙江杭州310053

出　　处：《中华中医药学刊》2013年第10期2221-2223,I0005,共4页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省自然科学基金资助项目(LQ12H28003);浙江省中医药重点研究资助项目(2011ZZ003);浙江省大学生科技创新资助项目(2011R410033);浙江中医药大学科技资助课题(2012ZR07)

摘　　要：目的:采用中心组合(Box-Behnken)设计优选南方红豆杉[Taxus mairei(Lemee et Levl.)S.Y.Hu ex Liu]枝条中多糖的微波提取工艺。方法:在单因素实验基础上,采用Box-Behnken设计对影响南方红豆杉枝条中多糖提取率的主要因素(料液比、提取时间、提取次数)进行优化,建立影响因素与响应值之间的数学模型,确定最佳超声提取工艺条件。结果:南方红豆杉枝条中多糖最佳超声提取工艺为:料液比21.48(V/W),提取时间13.78 min,提取次数3次,微波频率为1887MHz。结论:采用响应面法对南方红豆杉中多糖的提取条件进行优化,该方法合理可靠,能为生产工艺提供理论依据。Objective ： The Box - Behnken was selected to optimize the extraction technology of polysaccharides from the branches of Taxus mairei （ Lemee et Levi. ） S. Y. Hu ex Liu. Method： Based on the single factor experiment, the main elements （ the ratio between liquid and solid, the extractive time and times） affecting the extraction rate of polysaccharides from the branches of Taxus mairei（ Lemee et Levi. ） S. Y. Hu ex Liu were optimized by using response surface analysis, and the functional relationship between the response value and elements was established. Result ： The result of the experi- ment showed the optimal extraction conditions were as follows ：21.48 times of water, extracting 13.78 min by 1887 MHz microwave frequency,3 times. Conclusion： It is feasible and reliable to optimize the conditions of extracting the polysac- charides from the branches of Taxus mairei （Lemee et Levl. ） S. Y. Hu ex Liu by response surface analysis which lays the theoretical foundation to its production process.

关 键 词：南方红豆杉 多糖 微波提取 BOX-BEHNKEN设计 

分 类 号：R284.3[医药卫生—中药学]