双酚A对萼花臂尾轮虫生殖的影响  

Effect of bishphenol A on the reproduction of the rotifer Brachionus calyciflorus

作　　者：陆正和[1,2] 阎斌伦[1,2] 杨家新[3] 

机构地区：[1]淮海工学院海洋学院,江苏连云港222005 [2]江苏省海洋生物技术重点建设实验室,江苏连云港222005 [3]南京师范大学生命科学学院,江苏南京210046

出　　处：《中国环境科学》2013年第10期1850-1855,共6页China Environmental Science

基　　金：江苏省海洋海洋生物技术建设实验室项目(2009HS15);国家自然科学基金资助项目(30570260)

摘　　要：应用生态毒理学研究方法，研究了不同浓度双酚A（BPA）对萼花臂尾轮虫生殖的影响，内容包括：2d的种群增长率（r）,3d的混交雌体数/非混交雌体数（MF／AF）、混交率（MR）、雄体数／雌体数（M／F）、携卵雌体数／非携卵雌体数（OF／NOF）、受精率（FR），7d的休眠卵产量（RE）及2d的休眠卵孵化率（Hit）.结果显示，与对照组相比，BPA0.5mg／L及以上浓度显著降低轮虫2d的，值（0.83～0.86）；BPA对轮虫3d的M／F及OF／NOF无显著影响：但BPA 0.25～1.0mg／L显著降低MF／AF、MR,BPA0.125mg／L及以上浓度显著降低FR（3.33％～15.33％）和RE（25.2～39.2ind/8mL）；BPA 0.0625～1.0mg／L显著降低HR（8.0％～22.0％）.因此，既然BPA对测试的轮虫生殖具有负面影响，为保护浮游动物，其在自然水体中浓度必须监控.Using ecotoxicological approach we studied the effect of bishphenol A （BPA） on the reproductive variables of B calyciflorus including population growth rate （r）, and the mictic female/amicic female （MF/AF）, mixis rate （MR）, total male number/total female number （M/F）, ovigerous females/non-ovigerous females （OF/NOF）, fertilization rate （FR）, resting egg production （RE）, and cyst hatching rates. The results showed that BPA at 0.5mg/L or higher concentration significantly decreased r （0.83-0.86） to control, but BPA had no significant effect on M/F and OF/NOF. BPA from 0.25to 1.0mg/L reduced markedly MF/AF and MR. BPA at 0.125 to 1.0mg/L decreased significantly fertilization rate （3.33%-15.33%） and resting egg production （25.2-39.2ind/8mL）. BPA at 0.0625to 1.0mg/L also significantly reduced the hatching rates （8.0%-22.0%） of resting eggs.The results showed that different endpoints of reproductive parameters had different sensitivities to BPA. HR of rotifers appeared to be most sensitive to the toxicity by BPA. Thus, since BPA had an adverse effect on the reproduction of the tested rotifer species, its concentrations in natural waterbodies must be monitored to protect the zooplankton.

关 键 词：萼花臂尾轮虫 双酚A 生殖 混交雌体 生态毒理学 种群增长率 BPA 中浓度 

分 类 号：Q959.181[生物学—动物学]