HPLC同时测定虎耳草中的岩白菜素、原儿茶酸和没食子酸的含量  

Sensitive determination of bergenin,protocatechuic acid and gallic acid in saxifrage by HPLC

作　　者：秦红英[1] 周光明[1] 彭贵龙[1] 李俊平[1] 

机构地区：[1]西南大学化学化工学院发光与实时分析教育部重点实验室,重庆400715

出　　处：《四川大学学报（自然科学版）》2013年第5期1044-1048,共5页Journal of Sichuan University(Natural Science Edition)

基　　金：国家自然科学基金(21277110)

摘　　要：建立了以离子液体1-丁基-3-甲基咪唑六氟磷酸盐([BMIm]PF6)为萃取剂,结合超声辅助萃取,利用高效液相色谱法同时分离测定虎耳草中的岩白菜素、原儿茶酸和没食子酸三种有效成分的方法.方法:采用Phenomenex C18色谱柱(250mm×4.6mm,5μm);流动相为甲醇-0.2%冰醋酸水溶液(20∶80,V/V);流速0.8mL/min;紫外检测波长273nm;柱温为35℃.结果:该方法对岩白菜素、原儿茶酸和没食子酸分别在0.00156～130μg/mL(r=0.99999),0.00172～143μg/mL(r=0.99988),0.00140～116μg/mL(r=0.99993)范围内线性关系良好,相关系数(r)均大于0.9998,定性检测限(S/N=3)依次是:0.1295ng/mL,0.0749ng/mL,0.0277ng/mL;样品回收率为91.10%～98.26%.结论:本法操作简单快速、定量准确、灵敏度高、成本低、且对环境友好,为虎耳草中岩白菜素、原儿茶酸和没食子酸的检测及分离提供了一个有效的科学方法.A simple high performance liquid chromatography （HPLC） method has been developed for the simultaneous quantification of bergenin, protocatechuic acid and gallic acid from saxifrage using 1-butyl- 3-methylimidazolium hexafluorophosphate as extractant with ultrasonic assisted extraction. Methods. Three compounds were isolated by using a Phenomenex C18 column in methanol --0.2% acetic acid so lution （20 ： 80, v/v） with the flow rate of 0.80 mL/min and the UV detection wavelength at 365 nm. Results： The linearity range for bergenin, protocatechuic acid and gallic acid were found to be 0. 00156- 130 μg/mL, 0. 00172-143 μg/mL, and 0. 00140-116 μg/mL respectively, and the correlation coefficients 0. 99999, 0. 99988 and 0. 99993, respectively. It was indicative of good linear dependence of peak area on concentration. The limits of detection （LOD） are 0. 1295 ng/mL, 0. 0749 ng/mL and 0. 0277 ng/mL, respectively. Accuracy of the method was checked by conducting recovery studies at three different levels for all the three compounds and the average percentage recoveries were ranged from 91. 10% to 98.26%. Conclusion： The proposed method was found to be simple, precise, specific, sensitive and accuracy. It provides a new and scientific means for routine quality control of saxifrage.

关 键 词：离子液体 超声辅助 高效液相色谱 虎耳草 

分 类 号：O652.62[理学—分析化学]