Preparation of monoclonal antibody to P53 and its clinical application  

P53单克隆抗体的制备及其临床应用(英文)

作　　者：Wenqing Wei Junhua Wu Jing Liu Yuxia Wang 

机构地区：[1]General Hospital of Beijing Military Area Command [2]Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences

出　　处：《The Chinese-German Journal of Clinical Oncology》2013年第10期473-476,共4页中德临床肿瘤学杂志（英文版）

基　　金：Supported by grants from the National Natural Science Foundation of China(No.30973562);National Basic Research Program of China(No.2010CB933904)

摘　　要：Objective：The aim of this study was to prepare monoclonal antibody against P53, a kind of tumor suppressor protein,and use the antibody initial y in clinical immunoassay. Methods：Monoclonal antibody was prepared and identified via the classic protocol of monoclonal antibody preparation. Identified monoclonal antibodies were purified by af inity chro-matography. Antibody titer was determined by enzyme linked immunosorbent assay （ELISA）. The specific binding activity of antibody was detected by Western blotting and immunohistochemistry. Results：Three strains of monoclonal antibodies named 1P15, 2P37 and 3P40 were obtained and purified by af inity chromatography. The purity of antibodies was higher than 90%. The titers of antibodies were more than 1：6000. Western blot and immunohistochemistry assay showed that the specific antibody can combine with endogenous P53 protein in the tumor celllines and determine the expression of P53 in tumor tis-sue. Conclusion：Three strains of monoclonal antibodies with high af inity to P53 were successful y established, which can be used for detecting the expression of P53 in tumor cells or tissue.

关 键 词：P53 protein monoclonal antibody tumor 

分 类 号：R392[医药卫生—免疫学]