抗条锈病小偃麦渗入系的HMW-GS组成分析  

Composition of HMW-GS in Wheat-alien Introgression Lines Resistant to Stripe Rust from Tritileytrigia

作　　者：王乐[1] 张晓军[2] 郭慧娟[2] 乔麟轶[2] 詹海仙[2] 李欣[2] 畅志坚[2] 孙美荣[3] 

机构地区：[1]山西大学生物工程学院,山西太原030006 [2]山西省农业科学院作物科学研究所,山西太原030032 [3]山西省农业科学院谷子研究所,山西长治046011

出　　处：《山西农业科学》2013年第10期1027-1030,共4页Journal of Shanxi Agricultural Sciences

基　　金：国家自然科学基金项目(31171839);山西省国际科技合作计划项目(2012081006-2;2013081007);山西省科技攻关项目(20130311001-5);山西省回国留学人员科研资助项目(2012-102)

摘　　要：利用SDS-PAGE技术对101份来源于中间偃麦草的高抗或免疫条锈病的小偃麦渗入系的HMW-GS组成进行分析。结果表明,其存在丰富的HMW-GS等位基因变异类型;Glu-A1位点出现了1,2*,N共3种变异类型,Glu-B1位点出现了7,7+8,7+9,13+16,13+19,14+15,17+18共7种变异类型,Glu-D1位点出现了2+12,5+10,2+10,5+12共4种变异类型;其中含有对改良小麦加工品质有重要影响的17+18亚基的材料有15份,含有14+15亚基的材料有2份,含有5+10亚基的材料有14份。以这些材料作为亲本,对我国小麦品质育种及抗病育种可能具有重要的价值。The composition of HMW glutenin subunits were analyzed by SDS-PAGE for 101 wheat-alien introgression lines highly resistant to stripe rust from Thinopyrum intermedium. Rich HMW-GS allelic variants have been found. Three allelic variants of Null, 1 and 2＊ were detected at Glu-A 1 loci. Seven alleles, including the high-quality scored subunits of 14 ＋ 15, 17 ＋ 18 and 13 ＋ 16, were detected at the Glu-B1 loci. While four alleles for the Glu-D1 loci were detected including 2 ＋ 12, 5 ＋ 10, 2 ＋ 10, 5 ＋ 12. Fifteen varieties containing 17 ＋ 18 subunit and two varieties containing 14 ＋ 15 subunit and fourteen varieties containing 5 ＋ 10 were detected and might affect the improvement of wheat processing and baking quality. This will provide useful parents for wheat quality and stripe rust resistance breeding.

关 键 词：小偃麦 渗入系 条锈病 高分子量谷蛋白亚基 品质育种 

分 类 号：S512.9[农业科学—作物学]