基于斜投影算法对混合染液不展开多波长薄层色谱定量分析  

Oblique projection-based not spread multi-wavelength thin layer chromatography quantitative analysis of mixed dye

作　　者：葛军[1] 姚志湘[1] 粟晖[1] 宋光均 高玲 迟茹[1] 

机构地区：[1]广西工学院生物与化学工程系,广西柳州545006 [2]广州标旗电子科技有限公司,广东广州510640

出　　处：《印染助剂》2013年第10期47-50,共4页Textile Auxiliaries

摘　　要：将多波长薄层扫描和化学计量学相结合，应用斜投影法对混合染料中阳离子黄X-5GL和X-8GL进行分析．斜投影法利用投影将被测向量光谱信号从混合光谱信号中分离出来，对分离的信号进行计算分析以获得被测组分的含量．结果表明：2种染料点样量在0．5-2．5μg时，点样量与吸光度积分值具有良好的线性关系，相关系数R分别为0．9994、0．9995；所测得的混合染液中阳离子黄X-5GL和X-8GL的RSD较多波长薄层展开定量法小．Cationic yellow X-5GL and X-8GL mixed dye was analyzed using the multi-wavelength thin layer scanning and chemometrics through oblique projection method. Oblique projection method could sepa- rate quantity spectral signal from mixed spectrum signal using projection, and calculate and analyze the sepa- rated signal to obtain the content of measured component. The results showed that when two kinds of dye sample quantity range was 0.5-2.5 μg, the sample quantity and absorbance integral value had a good linear re- lationship, and the correlation coefficient R were 0.999 4 and 0.999 5, respectively. The measured RSD of cat- ionic yellow X-5GL and X-8GL in the mixed dye solution were lower than the traditional TLC method.

关 键 词：多波长薄层扫描 定量分析 斜投影 阳离子染料 

分 类 号：TQ610.7[化学工程—精细化工]