Mutagenesis of Arachidonic Acid-producing Mortierella Isabellina and Analyses of Δ~6-desaturase Role by qPCR  

作　　者：Yao Di Yu Chang-qing Yang Jian Li Li-na 

机构地区：[1]College of Food, Heilongjiang Bayi Agricultural University [2]Quality Supervision and Inspection Center of Agricultural Processed Products on Agriculture Ministry

出　　处：《Journal of Northeast Agricultural University(English Edition)》2013年第3期63-70,共8页东北农业大学学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China(31171657);Heilongjiang Youth Science Foundation(QC2010093);Heilongjiang Natural Science Foundation(20100481037);Key Projects on Ministry of Education in Heilongjiang(12511Z022)

摘　　要：Arachidonic acid （AA or ARA）, an essential to-6 polyunsaturated fatty acid （PUFA）, can be produced by Mortierella isabellina. Mutagenesis on Mortierella isabellina As3.3410 was induced to raise ARA production. The mutant strain of YZ-124 had the highest ARA of 4.72 g. L-1, which was 5.5 times higher than that of the original strain 3.3410. mRNA expression level of △ 6- desaturase was determined in five different kinds of ARA-producing Mortierella isabellina after cultured for 7 days, and in the mutant strain YZ-124 over a 3-8 day time-course. In addition, the desaturase activity and ARA content were measured at the selected time points. The lowest expression of △6-desaturase was observed in the original strain and the highest expression in the mutant strain YZ-124, which increased with increasing time in culture. Furthermore, a positive correlation was observed between the expression levels of △6-desaturase and ARA content. Based on this, △6-desaturase played a significant role in ARA synthesis pathway in Mortierella isabellina.Arachidonic acid （AA or ARA）, an essential to-6 polyunsaturated fatty acid （PUFA）, can be produced by Mortierella isabellina. Mutagenesis on Mortierella isabellina As3.3410 was induced to raise ARA production. The mutant strain of YZ-124 had the highest ARA of 4.72 g. L-1, which was 5.5 times higher than that of the original strain 3.3410. mRNA expression level of △ 6- desaturase was determined in five different kinds of ARA-producing Mortierella isabellina after cultured for 7 days, and in the mutant strain YZ-124 over a 3-8 day time-course. In addition, the desaturase activity and ARA content were measured at the selected time points. The lowest expression of △6-desaturase was observed in the original strain and the highest expression in the mutant strain YZ-124, which increased with increasing time in culture. Furthermore, a positive correlation was observed between the expression levels of △6-desaturase and ARA content. Based on this, △6-desaturase played a significant role in ARA synthesis pathway in Mortierella isabellina.

关 键 词：Mortierella isabellina △6-desaturases arachidonic acid real-time PCR 

分 类 号：S512.1[农业科学—作物学]