组织芯片制备及在肝癌研究中的应用体会  

Tissue microarray construction and application experiences in Hepatocellular carcinoma research

作　　者：余杏娟[1] 许静[1] 郑利民[1] 

机构地区：[1]华南肿瘤学国家重点实验室中山大学肿瘤防治中心实验研究部,广州510060

出　　处：《中国免疫学杂志》2013年第10期1089-1092,共4页Chinese Journal of Immunology

基　　金：卫生部重大专项基金资助项目(2012ZX10002-011);十二五重大科技专项资助

摘　　要：目的:探讨采用全自动组织芯片仪进行组织芯片制作,以及组织芯片在肝癌研究中的应用体会。方法:利用组织芯片仪制备肝癌组织芯片16枚,共包含313例肝癌患者肝癌组织。通过免疫组化方法检测了HLA-DR抗体,并比较其在肝癌癌旁及肿瘤内的表达情况。结果:采用全自动组织芯片仪能更加完好地构建肝癌组织芯片。用此组织芯片检测HLA-DR抗原在肝癌中高阳性表达率为51.1%,而在癌旁组织为76.4%,两者具有统计学差异(P<0.000 1)。结论:全自动组织芯片仪制备的组织芯片能简单、快捷检测相关蛋白在肝癌组织中的表达。组织芯片在肝癌研究中具有高效、快速、方便、经济等特点。Objective：To apply automation tissue an＇ay building system in tissue microarray （TMA） construction and explore the application value of TMA technology in hepatocellular carcinoma （HCC） research. Methods： TMA blocks which containing 313 biopsies of HCC tissue were prepared using automation tissue array building system. The expression of HLA-DR protein was examined by conven- tional immunohistoehemistry technique. Results： Sixteen TMA blocks were successfully constructed. HLA-DR protein was analyzed in these TMAs and was found highly expressed in peri-tumoral tissues （51.1%） of HCC as compared with intra-tumoral tissues （76. 4% ; P 〈 0. 000 1 ）. Conclusion： TMAs can be simply and quickly constructed with automation tissue array building system. TMA techniques are useful in HCC research with the advantages of high-throughput, rapid, simple, economic and equivalent applications.

关 键 词：组织芯片 肝癌 HLA—DR 

分 类 号：R392-33[医药卫生—免疫学]