环氧化酶-2与单核-内皮细胞黏附性的相关研究  

The research of the relationship between Cyclooxygenase-2 and monocyte-endothelial cells adhesion

作　　者：王红艳[1] 徐春亮[2,3] 蔡凡[1] 曹久妹[1] 陈晓南[1] 吴方[1] 

机构地区：[1]上海交通大学医学院附属瑞金医院老年病科,上海200025 [2]上海交通大学医学院 [3]中国科学院上海生命科学研究院健康科学研究中心,上海200025

出　　处：《中国实验诊断学》2013年第10期1743-1746,共4页Chinese Journal of Laboratory Diagnosis

基　　金：上海市科委基金(124119a6800);上海市教委基金(11YZ57)

摘　　要：目的探讨环氧化酶-2(Cyclooxygenase-2,COX-2)表达水平同单核-内皮细胞黏附性的关系及其相关机制。方法采用细胞计数法检测单核-内皮细胞的黏附性;实时荧光定量PCR检测内皮细胞COX-2mRNA表达量;Western blot检测内皮细胞COX-2蛋白表达量;ELISA检测内皮细胞前列腺素E2(Prostaglandin E2,PGE2)水平。结果内皮细胞COX-2mRNA及蛋白表达量在炎症因子TNF-α、IL-1β刺激后显著上调,并随时间变化(P<0.05);内皮细胞COX-2蛋白表达量增加与PGE2水平及单核-内皮细胞的黏附性成正相关;使用COX-2特异性抑制剂NS398预孵育后,内皮细胞PGE2表达水平降低(P<0.05),单核-内皮细胞的黏附性受到显著抑制(P<0.01)。结论内皮细胞COX-2mRNA及蛋白表达量在炎症因子TNF-α、IL-1β刺激后明显上调,其表达增加后可能通过催化花生四烯酸代谢成PGE2,使单核-内皮细胞的黏附性增强,从而影响动脉粥样硬化性疾病的进程。Objective To investigate the relationship between Cyclooxygenase-2 levels and monocyte-endothelial cells adhesion. Methods Monocyte-endotheliat cells adhesion was assessed by counting cells number. The expression of COX-2 mRNA and protein were measured by real-time PCR and Western blot assay. The expression of Prostaglandin E2 （PGE2） was performed by ELISA. Results Inflammatory cytokines TNF-α、IL-1β could significantly induce the ex- pression of COX-2 mRNA and protein in varying degrees（P〈0.05） ; High COX-2 levels could result in high PGE2 level and induce monocyte-endothelial cells adhesion signifieantly;COX-2 specific inhibitor NS398 significantly inhibited the inflammatory cytokines induced PGE2 expression （P〈0.05）,then affecting monoeyte-endothelial cells adhesion （P〈 0.01）. Conclusion Inflammatory cytokines TNF-α、IL-1β could induce the expression of COX-2, which could induce the expression of PGE2 ,monocyte-endothelial cells adhesion and then could affect the progress of the atheroselerotic dis- ease.

关 键 词：动脉粥样硬化 环氧化酶-2 人主动脉内皮细胞 单核-内皮细胞黏附 前列腺素E2 

分 类 号：R543.5[医药卫生—心血管疾病]