对比研究ELISPOT与QFT-GIT对于结核病诊断的应用价值  

To compare of ELISPOT and QFT-GIT assay for the diagnosis value of tuberculosis

作　　者：张娟[1] 印璞[2] 孙炳奇[1] 李莹[1] 张俊仙[3] 马静红[1] 孙秀华[1] 

机构地区：[1]沈阳市胸科医院,辽宁沈阳110044 [2]吉林省临床检验中心,吉林长春130021 [3]解放军第三0九医院全军结核病研究所,北京100091

出　　处：《中国实验诊断学》2013年第10期1839-1842,共4页Chinese Journal of Laboratory Diagnosis

基　　金：沈阳市科技计划项目(F11-262-9-38)

摘　　要：目的比较ELISPOT和Quanti-FERON-TB Gold In-Tube(QFT-GIT)试剂盒,探讨ELISPOT方法在活动性肺结核诊断中的意义。方法应用ELISPOT和Quanti-FERON-TB Gold In-Tube(QFT-GIT)对105份活动性结核患者(A组)和60份非结核呼吸系统疾病对照组(B组)进行检测。结果 ELISPOT与QFT-GIT方法的敏感度、特异度、阳性预测值及阴性预测值分别为85.71%、86.67%、91.84%及77.61%和83.81%、76.67%、86.27%及73.02%,且两种方法差异无统计学意义(均P>0.05)。结论应于ESAT-6/CFP-10融合蛋白技术的ELISPOT检测方法能够诊断活动性肺结核,并对结核感染诊断提供一定的依据。Objective To compare ELISPOT and Quanti-FERON-TB Gold In-Tube（QFT-GIT） assay and explore the application of Enzyme-linked immunosorbent assay（ELISPOT） in diagnosis of active pulmonary tuberculosis. Methods The two methods of IGRAs,Quanti-FERON-TB Gold In-Tube（QFT-GIT） and ELISPOT,was employed to detect 105 cases with active tuberculosis （A group） and 60 cases with non-TB pulmonary disease （B group）. Results The sensitivity, specificity, positive predictive value and negative predictive value ELISPOT. TB and QFT-GIT were re- spectively 85.71%,86.67%,91.84%,77.61% and 83.81%,76.67%,86.27%,73.02%. There were not statistically significance difference between two methods （P〉0. 05）. Conclusion Enzyme-linked immunosorbent assay（ ELIS- POT） using ESAT-6/CFP-10 fusion protein（E/C） can be aassistant to diagnosis active tuberculosis. It offers certain di- agnostic evidences for latent tuberculosis infection.

关 键 词：结核分枝杆菌 酶联免疫斑点实验 Quanti—FERON—TB GOLD In—Tube 

分 类 号：R52[医药卫生—内科学]