激光诱导纳秒时间分辨荧光法原位测定吸附于红树叶片表面的菲  

In situ Determination of Phenanthrene Adsorbed onto Surface of Mangrove Leaves Using a Laser-Induced Nanosecond Time-Resolved Fluorescence System

作　　者：杨亚男[1] 孙海峰[2] 朱亚先[1] 吴芳[2] 张勇[2,3] 

机构地区：[1]厦门大学化学系,厦门361005 [2]厦门大学环境科学研究中心,近海海洋环境科学国家重点实验室,厦门361005 [3]漳州职业技术学院,漳州363000

出　　处：《分析化学》2013年第10期1465-1469,共5页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(Nos.21075102;21177102);厦门大学基础创新科研基金;中央高校基本科研业务费专项资金(No.CXB2011036);国家基础科学人才培养基金(No.J1030415)资助项目

摘　　要：实现吸附于植物叶片表面多环芳烃（Polycyclicaromatichydrocarbons，PAHs）的现场原位测定，是该研究领域的发展方向之一。本实验利用激光诱导纳秒时间分辨荧光（Laser—inducednanosecondtime—resolvedfluorescence，LITRF）系统，建立了原位测定吸附于秋茄（Kandeliaobovata，Ko）、木榄（Bruguieragymnorhiza，融）和桐花树（Aegicerascorniculatum，Ac）3种红树叶片表面菲（Phenanthrene，Phe）的新方法。本方法测定吸附于K0、Bg和4c叶片表面Phe的线性范围分别为2—1400ng/spot，1—1000ng/spot和4～2000rig／spot，检测限分别为0．20，0．14和0．42ng／spot，加标回收率为89．6％～108．1％，78．2％～92．4％和93．2％～112．9％．且方法的相对标准偏差小于6．0％（n=9）。将方法用于实验室暴露样品的原位测定，并与光纤荧光法对比，其灵敏度、线性范围改善显著，更有利于实现植物叶片上PAHs的现场原位测定。Developing new methods for in situ determination of polycyclic aromatic hydrocarbons （PAHs） in plant leaves is of great significance to further achieve the field detection of PAHs in actual leaf samples. A set of laser-induced nanosecond time-resolved fluorescence （LITRF） system was employed to complete the in situ quantitative determination of the phenanthrene （Phe） that was adsorbed onto the leaf surfaces of three kinds of mangrove species, named Kandelia obovata （Ko）, Bruguiera gymnorhiza （Bg） and Aegiceras corniculatum （Ac）. Experimental results showed that the linear dynamic ranges for the in situ determination of the Phe that was adsorbed onto the leaf surfaces of Ko, Bg and Ac were 2- 1400 ng/spot, 1- 1000 ng/spot and 4- 2000 ng/spot, with the detection limits of 0.20, 0.14 and 0.42 ng/spot, respectively. The relative standard deviations were less than 6.0% （ n = 9 ）. The experimental recoveries for the Phe in Ko, Bg, and Ac were 89.6%-108.1% , 78.2%-92.4% and 93.2% -112.9% , respectively. Satisfactory results were obtained for the in situ determination of the Phe that was adsorbed onto the surfaces of the lab-exposure mangrove leaf samples contaminated by the Phe. The sensitivity of the established method was much higher than that of the previously established fiber optical fluorimetry, and the linear dynamic range got improved. The LITRF method marked a significant step toward realizing the field detection of PAHs in vegetation.

关 键 词：激光诱导纳秒时间分辨荧光 原位 红树叶片 菲 

分 类 号：X830.2[环境科学与工程—环境工程] O657.3[理学—分析化学]