骨髓间充质干细胞对大鼠佐剂性关节炎防治的实验研究  

Research on the treatment and intervention of bone marrow mesenchymal stem cell to adjuvant arthritis in rats experiment

作　　者：许键炜[1] 申长清[2] 赵芳芳[3] 舒莉萍[4] 何志旭[4] 

机构地区：[1]贵阳医学院药理学教研室,550004 [2]济宁医学院附属医院儿科,山东济宁272029 [3]蚌埠医学院微生物学教研室,安徽蚌埠233030 [4]贵阳医学院组织工程与干细胞实验中心,550004

出　　处：《重庆医学》2013年第29期3472-3475,共4页Chongqing medicine

基　　金：国家自然科学基金资助项目(30560159);贵阳医学院基金资助项目(K2009-37)

摘　　要：目的探讨骨髓间充质干细胞(MSCs)对大鼠佐剂性关节炎(AA)的治疗、干预作用。方法收集培养扩增SD大鼠骨髓MSCs。SD大鼠注射弗氏完全佐剂造模之后行MSCs治疗及干预。造模后定期测量大鼠体质量、踝关节周长和后足爪体积。治疗后第21天,行四肢关节X线片及病理检查。结果模型大鼠经MSCs治疗后关节肿胀减轻,X线片示骨质破坏明显修复。病理HE染色见关节滑膜病变减轻。干预组经MSCs治疗,原发性炎症和继发病变均不明显,关节X线片和病理检查均未见明显异常,与模型组大鼠相比,体质量、足爪体积以及踝关节周长差异有统计学意义(P<0.05)。结论 MSCs治疗可有效减轻并逆转弗氏完全佐剂诱导的大鼠AA的进展。Objective To explore the mechanisms of action about how marrow mesenchymal stem cells （MSCs） treat and inter-vene the adjuvant arthritis （AA） of rats .Methods MSCs of SD rats which had been cultured and cloned would be collected .Fre-und′s complete adjuvant was administered in rats .The rats from the model group were given MSCs treatment and intervention .Dur-ing the treatment period ,those rats′ weights ,ankle circumferences and hind paw volumes were measured .On the 21st day of the treatment ,limb-joints X-ray examination and pathological examination were taken .Results After using MSCs to treat adjuvant ar-thritis of rats ,the joint swelling of the MSCs group were relieved ,and the limb-joints X-ray showed the destructive bones had ap-parent restoration .Pathological microscopic examination of HE staining ankle joint showed joint synovial lesions significantly reduced . In the MSCs intervention group ,rats′primary inflammation and secondary lesions were not obvious ,meanwhile ,joint X-ray and pathological examination showed no obvious abnormalities .Compared with the model group ,the differences of rats′weight ,paw volume and ankle circ-umference had statistically significant （P〈0 .05） .Conclusion MSCs could relieve and reverse the progression of AA .

关 键 词：骨髓间充质干细胞 关节炎 实验性 治疗 预防 

分 类 号：R593.22[医药卫生—内科学]