大黄酸及大黄素对人肾小管上皮细胞增殖及TGF-β_1启动子活性的调控作用  

Regulatory Effect of Rhein and Emodin on Proliferation of Human Renal Tubular Epithelial Cell Line and Activity of TGF-β_1gene Promotor

作　　者：杨君[1] 秦英[2] 赵斐[2] 杜树山[3] 于海[4] 

机构地区：[1]首都医科大学附属北京中医医院特需科,北京100010 [2]北京中医药大学东直门医院肾病内分泌科,北京100700 [3]北京师范大学教育部资源药物中心,北京100875 [4]中国人民解放军海军总医院肾内科,北京100037

出　　处：《上海中医药大学学报》2013年第5期70-72,81,共4页Academic Journal of Shanghai University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(30973737)

摘　　要：目的:观察大黄酸和大黄素对人肾小管上皮细胞(HK-2)增殖及对人转化生长因子-β1(TGF-β1)基因启动子活性的作用。方法:应用MTT法观察不同浓度大黄酸和大黄素(1μg/ml、10μg/ml、50μg/ml和100μg/ml)对人肾小管上皮细胞增殖的作用;将人TGF-β1基因启动子片段与氯霉素乙酰基转移酶(CAT)报告基因组成重组体ph TGF 2.14,采用脂质体法转染至人肾小管上皮细胞中,分别加入不同浓度的大黄酸和大黄素(1μg/ml、10μg/ml和50μg/ml)进行干预,用ELISA方法检测报告基因CAT的活性。结果:大黄酸和大黄素各浓度组对人肾小管上皮细胞均具有明显的抑制HK-2细胞增殖的作用(P<0.01),且呈剂量依从性;大黄酸和大黄素浓度为1μg/ml和10μg/ml时对重组体ph TGF 2.14的表达活性无影响,当增大其浓度为50μg/ml时对重组体ph TGF 2.14的表达活性有抑制作用,其抑制率分别为38.0%和36.0%,与对照组比较均有显著差异(P<0.01)。结论:大黄酸和大黄素能够抑制人肾小管上皮细胞增殖,当浓度为50μg/ml时对人TGF-β1基因启动子活性具有一定的抑制作用。Objective ： To observe the effect of rhein and emodin on the proliferation of human renal tubular epithelial cell line （HK-2） and the activities of TGF-β1 gene promoter. Methods： Effects of rhein and emodin on proliferation of HK-2 were analyzed by MTT; different lengths of human TGF-β1 gene and CAT as reporter gene were constructed and were transferred into HK-2 RMC by transfection reagent. The effects of rhein and emodin （1 μg/ml, 10 μg/ml, 50 μg/ml and 100 μg/ml） on the plasmid were determined by CAT-ELISA. Results： Rhein and emodin of different concentrations had regulatory effect on the proliferation of HK-2 （P 〈0.01 ） , and with dose compliance; rhein and emodin at 1 μg/ml and 10 μg/ml didn＇t inhibit the activities of phTGF 2.14. Rhein and emodin at 50 μg/ml markedly inhibited the activities of ph TGF 2.14, and the inhibition ratio was 38.0% and 36.0%, was different from the control group （P 〈 0.01 ）. Conclusion： Rhein and emodin have regulatory effect on the proliferation of HK-2. Rhein and emodin at 50 μg/ml have regulatory effect on the activity of TGF-β1 gene promoter.

关 键 词：大黄酸 大黄素 转化生长因子-Β1 启动子 人肾小管上皮细胞 

分 类 号：R285.5[医药卫生—中药学]