溃疡方对乙酸胃溃疡大鼠黏膜TFF1、EGF和EGFR的影响  

Effects of Anti-ulcer Formula on TFF1,EGF and EGFR in Mucous Membrane of Rats with Acetic Acid Gastric Ulcer

作　　者：刘娜[1] 江伟[1] 

机构地区：[1]桂林医学院附属医院,广西桂林541002

出　　处：《河南中医》2013年第10期1649-1651,共3页Henan Traditional Chinese Medicine

摘　　要：目的:探讨溃疡方对乙酸性胃溃疡大鼠黏膜三叶肽因子1(TFF1)、表皮生长因子(EGF)和表皮生长因子受体(EGFR)的影响。方法:将50只SD大鼠随机分为5组,正常对照组、胃溃疡模型组、溃疡方低剂量组、溃疡方高剂量组、雷尼替丁组对照组,每组10只,雌雄各半。从术后第3天开始灌胃给药:溃疡方低、高剂量组(予溃疡方免煎剂,浓度为2 g/mL、4 g/mL),雷尼替丁组给予雷尼替丁30 mg,模型组及正常组均给予等量的生理盐水,每组均按1 mL/100 g,1次/d,连续14 d。拉颈处死大鼠,取胃组织,用逆转录聚合酶链反应(RT-PCR)技术检测胃黏膜组织中TFF1、EGF及其受体EGFR mRNA的表达和免疫组织化学法检测胃黏膜TFF1、EGFR的含量。结果:RT-PCR结果示:与正常组相比,模型组TFF1、EGF水平降低(P>0.05),溃疡方高剂量组及雷尼替丁组TFF1均显著升高(P<0.01),溃疡方各组及雷尼替丁组的EGF含量均高于正常组(P均<0.01),而模型组EGFR的含量较正常组升高(P>0.05),但溃疡方各组及雷尼替丁组升高更明显(P均<0.01);与模型组比较,溃疡方低剂量组、溃疡方高剂量组及雷尼替丁组TFF1、EGF及EGFR的表达均显著升高(P均<0.01);免疫组织化学结果显示:溃疡方各剂量组及雷尼替丁组TFF1及EGFR的表达均显著高于模型组(P<0.01),尤溃疡方高剂量组显著。结论:溃疡方对乙酸所致大鼠胃溃疡有防御和修复治疗作用,其作用机制可能是通过促进胃黏膜中TFF1、EGF和EGFR的表达有关。Objective ： To explore the effects of anti-ulcer formula on trefoil factor 1 （ TFF1 ）, epidermal growth factor （EGF） and epidermal growth factor receptor （EGFR） in mucous membrane of rats with acetic acid gastric ulcer. Methods： 50 SD rats were randomly divided into five groups, normal control group, gastric ulcer model group, anti-ulcer formula of low dosage group, anti- ulcer formula of high dosage group, ranitidine control group, 10 rats in each group （5 male rats and 5 female rats）. 3 days after the operation, gastric administration of drugs were given ： the anti-ulcer formula of low and high dosage groups were given decoc- ring-free anti-ulcer formula with respective density of 2g/ml and 4g/ml. Ranitidine group were given ranitidine 30rag. Both the model group and normal group were given nornral saline of equal amount, each group with lmL/100g, once per day for consecu- tive 14 days. For the rats died of pulling necks, their gastric tissues were taken out and the expression of m RNA of TFF1, EGF and EGFR in the gastric mucosa were detected by RT - PCR as well as the content of TFFI and EGFR in gastric mucosa by im- munohistochemical method （IHC）. Results： The results of RT- PCR showed that： compared with the normal group, the level of TFFI and EGF in model group decreased （ P 〉 0. 05 ） ; the level of TFF1 in anti-ulcer formula of high dosage group and raniti- dine group increased remarkably （ P 〈 0. 01 ） ; the content of EGF in each anti-ulcer formula and ranitidine group was higher than that in normal group （ P 〈0.01） ; the content of EGFR in model group increased compared with that of the normal group （ P 〉 0. 05 ） ; while the increase in each anti-ulcer formula and ranitidine group was much more noticeable （P 〈0. 01 ） ; the expression of TFF1, EGF and EGFR was significantly improved in anti-ulcer formula of low dosage group, anti-ulcer formula of high dosage group and ranitidine group（ P 〈 0. 01 ） , among which the increase in anti-ulcer fo

关 键 词：乙酸胃溃疡 溃疡方 三叶因子1 表皮生长因子 表皮生长因子受体 大鼠 

分 类 号：R285.5[医药卫生—中药学]