慢性乙肝中医证型的机体免疫反应状况分析  

Analysis of immune response conditions in chronic hepatitis B with different TCM syndromes

作　　者：魏嵋[1] 王晓栋[1] 肖斌[2] 刘友平[2] 

机构地区：[1]泸州医学院附属中医医院肝胆内科,四川泸州646000 [2]泸州医学院生物化学教研室,四川泸州646000

出　　处：《泸州医学院学报》2013年第5期427-431,共5页Journal of Luzhou Medical College

基　　金：四川省教育厅资助项目(09ZA048;13ZA0239)

摘　　要：目的:通过分析慢性乙肝5种中医证型患者外周血浆蛋白质的差异表达程度,初步探索慢性乙肝各中医证型间免疫反应状况规律。方法:采用双向电泳技术结合质谱对慢性乙肝5种中医证型患者(各30例)及正常对照组(10例)的血浆全蛋白进行比较蛋白质组学分析,然后扩大样本量,选取其中与肝脏损伤及机体免疫功能相关的差异表达蛋白质进行ELISA检测其变化规律。结果:①比较蛋白质组学分析发现在5种慢性乙肝中医证型中均存在明显规律性差异表达的蛋白点共有5个:(haptoglobin,HPT)、免疫球蛋白J链(immunoglobulin J-chains,IGJ)、视黄醇结合蛋白(retinol binding protein,RBP)、C-反应蛋白(C-reactive protein,CRP)、玻连蛋白(vitronectin,VN)。②ELISA检测发现在5种中医证型中与肝脏功能相关的蛋白质——RBP、HPT较正常对照组下调与机体免疫功能相关的蛋白质——IGJ较正常对照组上调的高低顺序是一致的。结论:慢性乙肝中医证型演变过程与机体体液免疫状态之间存在正相关性,肝脏病情越严重的中医证型其机体免疫功能越紊乱。Objective To explore the link between the immune response and the five types of traditional Chinese medicine （TCM） syndromes of patients with chronic hepatitis B （CHB） by proteomic analysis. Methods： Plasma was prepared from 150 cases of CHB patients with five different types of TCM syndromes and 10 cases of normal control.Two dimensional gel electrophoresis （2-DE） followed by matrix-assisted laser desorption/ionization time-of-flight MS （MALDI-TOF-MS） was used to pick out important protein spots which had different expressions among different TCM syndromes and normal controls, and three of these differentially expressed proteins which related to liver injury and immune function were quantitatively analyzed by ELISA from more plasma samples. Results： Five differential proteins were： immunoglobulin J-chains （ICJ）, C-reactive protein （CRP）, haptoglobin （HPT）, retinol binding protein （RBP） and vitronectin （VN）. And the upregulation tendency of IGJ which related to the immune function were consistent with the downregulation tendency of HPT and RBP which related to liver injury. Conclusion： The humora] immunization play a very important role in the process of the five TCM syndromes of CHB and the stronger humoral immune response is, the more severe liver damage is.

关 键 词：慢性乙肝 中医证型 免疫反应 蛋白质组学 

分 类 号：Q257[生物学—细胞生物学]