硝普钠(SNP)和茉莉酸甲酯(MeJA)诱导的白桦Ty1-copia类逆转座子的克隆及分析  

Clone and Analysis of Reverse Transcriptase of Ty1-copia-like Retrotransposons in Betula platyphylla under Sodium Nitroprusside( SNP) and Methyl Jasmonate( MeJA) Treatment

作　　者：梁楠松[1] 曾凡锁[1] 李博[1] 郭梁[1] 詹亚光[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2013年第10期69-74,共6页Journal of Northeast Forestry University

基　　金：国家自然科学基金(31070531);黑龙江省高校青年学术骨干计划项目(1155G08)

摘　　要：利用Tvl—copia类逆转座子逆转录酶（RT）的保守区域设计引物，优化了扩增条件后利用RT—PCR从白桦愈伤组织中得到了1条逆转录酶序列。利用硝普钠（SNP）和茉莉酸甲酯（MeJA）诱导后分别扩增克隆了11条和9条逆转录酶序列。这2l条逆转录酶序列（命名Bprtl-21）变化范围为205—290bp，一致性为71．93％，异质性为1．1％。50．9％，翻译成氨基酸的异质性为0～57．1％。经SNP和MeJA处理的样品中，获得的逆转录酶序列基本是分别聚类．初步证明不同的Tvl-copia类逆转座子能够分别响应不同的信号而被转录激活。分析所获得的21逆转录酶序列和苹果中19务逆转录转座子序列（DQ410748-DQ410766）进化关系发现．逆转录酶基本各自聚类。但是Bprt9、Bprtll和OQ4m750进化关系比较接近，Bprt15、Bprt19、DQ410751和DQ410753聚为一类，证明逆转座子存在水平传递。We designed the primers of reverse transcriptase according to the conserved domains of the Tyl-copia retrotransposons reverse transcriptase. We obtained several fragments of reverse transcriptase from callus of birch by using improved RT- PCR. Eleven and nine fragments were amplified from callus of birch treated by sodium nitroprusside （SNP） and methyl jasmonate （MeJA）, respectively. The length of 21 reverse transcriptase sequences （named Bprt 1-21 ） is from 205 to 290 bp, with the consistency of 71.93%, the heterogeneity of from 1.1% to 50.9% and the heterogeneity of amino acid se- quences of from 0.0% to 57.1%. The sequences from birch treated by SNP or MeJA are clustered, respectively. The transcription of Tyl-copia-like retrotransposons can be activated by different signals. The analysis of the phylogenetic tree of reverse transcriptase of Tyl-copia-like retrotransposons between birch and apple revealed each of the reverse transcripta ses are clustered together, but the evolutional relationship between Bprt9, Bprtll and DQ410750 are closer. Bprtl5, Bprtl9, DQ410751 and DQ410753 are clustered together. Therefore, the horizontal transmission can exist in retrotrans- posons.

关 键 词：白桦 Tyl-copia类逆转座子 逆转录酶 转录活性 系统进化 异质性 简并杂交寡核苷酸引物 

分 类 号：Q789[生物学—分子生物学]