虹彩病毒胁迫下东北林蛙NF-κB和I-κB的差异表达  

Expression Analysis of NF-κB and I-κB of RGV-Stressed Rana dybowskii

作　　者：周旋[1] 肖向红[1] 张晶钰[1] 柴龙会[1] 牛曙东[1] 吕晨[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2013年第10期108-111,共4页Journal of Northeast Forestry University

基　　金：国家自然科学基金项目(No.30870303);黑龙江省自然科学基金重点项目(No.ZJN0604-02)

摘　　要：根据实验室高通量测序所得核转录因子κB(NF-κB)NF-κB1、NF-κB2和I-κBα部分序列,应用荧光定量PCR技术,检测虹彩病毒(Rana grylio virus,RGV)胁迫下NF-κB1、NF-κB2以及I-κBαmRNA在东北林蛙(Rana dybowskii)肝脏、脾脏、皮肤中的差异表达,并对表达差异进行统计学分析。结果显示:在东北林蛙不同组织中,NF-κB1和NF-κB2的表达水平有差异,NF-κB1表达量约是NF-κB2的2倍,且皮肤和脾脏中的表达量远高于肝脏。在虹彩病毒刺激初,2个基因在3个组织中表达量立即下调,受刺激0.5 h后2个亚基在3个组织中都有上调趋势;肝脏和皮肤中,在刺激后2 h(在肝脏中)和4 h(在皮肤中)快速上调至峰值;在脾脏中,2亚基上调趋势缓慢,分别在16 h(NF-κB2)和24 h(NF-κB1)达到峰值;2亚基表达至峰值后开始下调,并在8 h(在肝脏中)和12 h(在皮肤中)下调至初始水平,在脾脏中2亚基72 h以后才达到初始水平。I-κBα在受刺激初期也立即下调而后上调,上调至峰值的时间点与NF-κB基本相同。统计分析结果表明:各实验组基因表达量与对照组存在统计学差异(P<0.05)。By high-throughput sequencing, the partial sequence of NF-KB1 ,NF- KB2 are two subunits of nuclear factor KB （NF- KB） and I-KBa. Under Rana grylio virus stress, we used real-time PCR to quantitatively detect NF-xB1, NF-KB2 and l-x.BamRNA in skin, liver and spleen of Rana dybowskii and statistically analyzed, the expression difference. The expression levels of the NF-KB1 and NF-KB2 have differences in different organizations, and NF-KB1 expression level is two times as high as NF-KB2. The expression level in skin and spleen is much higher than that in the liver. In the early stimulating stage, the expression of NF-KB1 and NF-KB2 is down-regulated immediately in three organizations. After 0.5 hour, two subunit expressions begin to increase. Expression rises quickly to the peak in the liver and skin-coerced for 2 hours （ in the liver） or 4 hours （in the skin） later. In the spleen, upward trend turns slow, and the maximum is at 16 hours （NF-KB2） or 24 hours （NF-KB1）. After reaching its peak, the expressions of two subunits decline again and they drop to the initial level at 8 hours （in the liver） or 12 hours （in the skin）, respectively. In the spleen, the initial level occurs after 72 hours. Ex- pression of 1-KBa is down-regulated immediately in the early stimulating. The raised time is substantially identical with that of NF-KB. By statistical analysis, there is statistically significant difference in the expression levels between experimental zrouD and control zroun （P 〈0.05 ）.

关 键 词：东北林蛙 虹彩病毒 NF-кB I-ΚB 荧光定量PCR 

分 类 号：Q7[生物学—分子生物学]