添加天然物质对白腐菌糙皮侧耳菌产漆酶的影响  

Influence of Natural Lixiviums in Liquid Medium on Pleurotus ostreatus Laccase Reproduction of Phanerochaete chrysosporium

作　　者：王辉[1] 陈广胜[1] 韩松[1] 

机构地区：[1]东北林业大学,哈尔滨150040

出　　处：《东北林业大学学报》2013年第10期112-115,共4页Journal of Northeast Forestry University

基　　金：国家自然科学基金面上项目(31170515);教育部新世纪优秀人才(NCET-09-0277);中央高校基本科研业务费专项资金(DL11EB02)

摘　　要：糙皮侧耳菌产漆酶的过程中,碳源葡萄糖质量浓度10 g/L,添加质量浓度为200 g/L的马铃薯浸出液,能够大幅提高漆酶产量,培养11 d漆酶产量达到112 U/mL。马铃薯浸出液在漆酶分泌阶段主要起到促进作用,并不提供碳源。进一步优化培养条件,转速150 r/min、28℃下,漆酶分泌最高;在25℃条件下,漆酶分泌量也较高,达到100 U/mL。In the Pleurotus ostreatus laccase reproduction of Phanerochaete chrysosporium, if the basic medium contains 10 g/L glucose as carbon source and 200 g/L lixivium of potato, the production of laccase after 11 days can observably reach 112 U/mL. The lixivium of potato mainly plays a promoting role in stead of providing a carbon source. When the speed is 150 r/min and the temperature is 28 ℃ the production of laccase secretion can reach the highest level. Laccase secretion can reach 100 u/mL at 25 ℃.

关 键 词：漆酶 白腐菌 糙皮侧耳菌 培养基 

分 类 号：Q93-335[生物学—微生物学]