微小隐孢子虫感染BALB/c小鼠肠道IL-17的变化  

Alteration of IL-17 in intestine of BALB/c mice infected by Cryptosporidium parvum

作　　者：黄清花[1] 赵魁[2] 李静 张巧灵[2] 刘波[1] 

机构地区：[1]吉林大学动物科学学院,吉林长春130062 [2]吉林大学动物医学学院,吉林长春130062

出　　处：《中国兽医学报》2013年第10期1559-1562,共4页Chinese Journal of Veterinary Science

基　　金：国家自然科学基金资助项目(30972170)

摘　　要：将24只3～4周龄雌性BALB／c小鼠随机均分为感染组和对照组（n=12），感染组小鼠接种浓度为1×10^6只／L的微小隐孢子虫卵囊液0．2mL／只，对照组接种0．2mL无菌生理盐水。在感染后7d和14d每组分别断颈处死6只小鼠，取空肠和结肠，对肠道组织培养上清中IL—17A及肠道IL-17A和IL17FmRNA表达水平进行检测。结果表明，与空白对照组相比，感染组BALB／c小鼠空肠和结肠中II．17A的表达量显著升高（P〈0．001）；感染组小鼠空肠中IL17FtuRNA表达水平也显著上升（P〈0．001）；在感染后7d，结肠IL—17F的表达量显著增加（P〈0．001），而感染后14dIL17F的rnRNA表达水平显著下降（P〈0．001）。Twenty-four female BALB/c mice were randomly selected and divided into infected and control group（n= 12）. The infected group were dosed orally with 0.2 mL of 1 ）〈 10G/L Cryptospo ridum parvum occyst,and control group mice were dosed orally with 0.2 mL isotonic Na chloride. Six mice were sacrificed on day 7 and 14 post infection each group, collected jejunum and colon samples to study the secretion of IL-17A in supernatants of gut culture and the alteration in mR- NA expression level of IL-17A and IL 17F in intestine. The results showed the expression of IL- 17A in jejunum and colon was increase significantly in BALB/c mice infected by Cryptosporidium parvum compared to control（P〈0. 001）. The mRNA level of IL-17F was up-regulated significant- ly in jejunum during Cryptosporidium parvum infection,however increased expression of IL-17F was observed in colon at 7 days post infection, and the mRNA level of IL-17F was makedlys de- creased on day 14 post infection in colon.

关 键 词：微小隐孢子虫 IL-17A IL 17F 

分 类 号：S852.723[农业科学—基础兽医学]