腹泻犊牛大肠杆菌血清型抗菌药物耐药性耐药基因鉴定及脉冲场凝胶电泳分型  

Serotypes,genotypes,antimicrobial resistance and resistant genes of Pathogenic Escherichia coli from diarrheic calves

作　　者：张陆[1] 蒋月[2] 盛鹏飞[2] 张秀英[1] 

机构地区：[1]东北农业大学动物医学学院,黑龙江哈尔滨150030 [2]辽宁医学院畜牧兽医学院,辽宁锦州121000

出　　处：《中国兽医杂志》2013年第9期15-17,共3页Chinese Journal of Veterinary Medicine

摘　　要：本试验对2011年由哈尔滨周边地区采集的263份腹泻犊牛的肛拭子进行大肠杆菌的分离鉴定,并通过小鼠致病性试验鉴定出26株致病性大肠杆菌,用Kirby-Bauer法开展对12种药物的药敏试验,PCR检测了我国流行广泛的9种耐药基因,脉冲场凝胶电泳(PFGE)进行基因分型。检测结果,该地区主要流行的致病性血清型为O26、O78和O15,所有致病性菌株都存在多重耐药现象,其中阿莫西林-棒酸、链霉素、四环素、复方新诺明的耐药率达到了100%,共检出7种耐药基因,检出率符合我国平均分布趋势,且检出率基本上与耐药表型相一致。PFGE结果显示,该地区存在致病性菌株的克隆传播。The aim of this study was to investigate the serotypes, genotypes, antimicrobial resistance and resistant genes in pathogenic Escherichia coli isolated from diarrheic calves around Harbin in 2011. A total of 263 rectal swabs were obtained from diarrheic calves, and 26 pathogenic E. coli strains were isola- ted from these samples. All the 26 pathogenic E. coli strains were tested with 12 antimicrobial agents with disk-diffusion method and characterize the 9 kings of resistant genes by PCR. High resistance to amoxicil- lin, streptomycin, tetracycline, trimethoprim-sulfamethoxazole had been detected. 7 kinds of resistant genes had been detected, and the positive rates were in accordance with the average tendency of China. 026, 078 and Ols were the main frequent serotypes. PFGE result demonstrated there was clonal dissemina- tion of pathogenic strains in this area.

关 键 词：大肠杆菌 耐药性 耐药基因 血清型 基因型 

分 类 号：S852.612[农业科学—基础兽医学]